Salicylic Acid Enhanced Photosynthesis, Secondary Metabolites, Antioxidant and Lipoxygenase Inhibitory Activity (LOX) in Centella asiatica
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 17, Issue 4
Abstract
Aims: The primary aim of this work was to investigate the impact of foliar application of SA on the leaf gas exchange, the accumulation of secondary metabolites and Lipoxygenase Inhibitory activity (LOX) in medicinal plant C. asiatica. Study Design: The study used randomized complete block design (RCBD) replicated three rwith three replications Place and Duration of Study: Department of Biology, Universiti Putra Malaysia between January 2014 and March 2014. Methodology: Cantella asiatica was propagated in individual poly bags filled with sand, coco dust and compost in the ratio of 1:1:1 and chicken dung (50 g) per poly bag. The plant was left for a month in a nursery to be acclimatized until they were ready for SA treatment. After a month, the plants were sprayed with two concentrations (1 µM and 10 µM) of salicylic acid solution (SA). Control plants (0 µM) were sprayed with same solution but without SA. The leaf gas exchange, total flavonoid, saponin, Glutathione (GSH), Oxidized Glutathione (GSSG), Phenyll alanine lyase (PAL), Chalcone synthase activity (CHS) and Lipoxygenase inhibitory activity (LOX) was measured. Results: It was found that production of secondary metabolites (total flavonoids and saponin), GSH, GSSG, PAL, CHS and LOX were solely contributed by interaction of SA levels and plant parts in the descending manner came in leaf treated with 10 µM SA> leaf-1 µM SA>leaf 0 µM SA> stem 10 µM SA> stem 1 µM SA> stem -0 µM SA> root 10 µM SA> root-1 µM SA > root 0 µM SA. High concentration of salicylic acid application (10 µM) was found to be more effective in enhancing the leaf gas exchange traits compared to low concentration (1 µM). Interestingly, it was found that increase in net photosynthesis have a high significant correlation with total flavonoid, total saponin, GSH, GSSG and LOX, indicating that up-regulation of photosynthetic capacity of C. asiatica treated with SA, enhanced the production of secondary metabolites, antioxidant and Lipoxygenase inhibitory activity (LOX). This study also showed that there was involvement of CHS and PAL activity in an increase in the production of secondary metabolites and antioxidant activities under SA application. Conclusion: It was found that SA enhances photosynthetic capacity of C. asiatica. This might be due to increase in PAL and CHS activity that was observed during the study. Furthermore, the production of secondary metabolites, antioxidant enzyme and Lipoxygenase inhibitory activity was elevated during the foliar SA application.
Authors and Affiliations
Mohd Hafiz Ibrahim, Hishamuddin Omar, Nurul Amalina Mohd Zain
Keywords
Related Articles
- EP ID EP313539
- DOI 10.9734/ARRB/2017/36153
- Views 102
- Downloads 0
Changes in Vascular Tissues and Productivity of Buckwheat Plants after Impulse Pressure Treatment
Osmotic pressure, pressure gradients in the phloem, and mechanical forces influence different processes in plants. We have proposed the method of pre-sowing seed treatment by impulse pressure generated by a shock wave. I...
Creek`s Aquaculture Techniques in Rangamati Hill Tracts of Bangladesh
An experiment was conducted in nine suitable (average .10±2 ha) creeks of Rangamati hill tracts Bangladesh viz., of Langadu, Naniarchar and Sadar Upazila for evaluating growth, survival and production performance of Indi...
Evolutionary Ecology of Diamondback Moth, Plutella xylostella (L.) and Diadegma insulare (Cresson) in North America: A Review
The diamondback moth (DBM), Plutella xylostella (L.), is recognized as a widely distributed destructive insect pest of Brassicaceae. The frequency and severity of P. xylostella outbreaks has increased in recent years, du...
Evaluation of Dietary Supplementation of Ammonium Sulphate on in vitro Gas Production and Rumen Fermentation Characteristics of WAD Ewes
Ammonium sulphate, a chemical compound was utilized to evaluate its potential through in vitro gas production technique and rumen fermentation characteristics as a rumen stimulator and mitigates methane gas. Four experim...
First Report on Colletotrichum acutatum of Olives in Morocco
Anthracnose is an important disease affecting the yield and quality of olive production (olives, olive oil). Symptoms of anthracnose, circular soft decayed spots were observed on olives in the region of Ouazzane (North o...