SALINOMYCIN INDUCES APOPTOSIS IN GERM CELLS OF MOUSE TESTIS
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 9
Abstract
Apoptosis is a strictly regulated process involving sequential activation of specific signal transduction pathway, disturbance of mitochondrial membrane function causing the release of intermembrane proteins into the cytosol and subsequent stepwise degradation of the cell. Adverse effect of Salinomycin on male reproductive organs and male infertility had been reported, however, the underlying mechanisms are still unclear. This study therefore investigates the possibility of Salinomycin-induced apoptosis through the interplay of bcl-2 and bax proteins in mouse testis. Eight groups of mice received 1, 3 and 5 mg/kg Salinomycin per day for a 28 days period. Four groups were sacrificed directly after the treatment while four groups were sacrificed 28 days after withdrawal of the treatment. Primarily, the role of apoptosis following Salinomycin exposure was evaluated using microarray profiling to assess effect of Salinomycin on essential genes in reproductive pathways. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling method (TUNEL) shows an increased number of positive staining germ cells in the testicles of Salinomycin-treated mice across the dose groups (< 0.05). Down regulation of Caspase 3 activity was also observed. Western blot analysis further reveals down regulation of bcl-2 protein with simultaneous up-regulation of bax apoptosis protein in testicles samples. A number of the regulated genes involved in reproductive pathways such as Adam 2, Spam, Tnp1, Tnp2, CYP11A1 and STRAD 6 are grossly affected. In general, microarray study reveals distinct expression profiles and patterns of regulated genes, dependent on the specific dose of Salinomycin. Conclusively, this study confirmed induction of apoptosis and further established dose dependent- adverse effects of Salinomycin on the male reproductive system.
Authors and Affiliations
Dr. Srikanta Kumar Rath
Keywords
Related Articles
- EP ID EP629001
- DOI -
- Views 187
- Downloads 0
YOGA DARSHANA AND AYURVEDA-A BIRD’S EYE VIEW
Yoga Darshana and Ayurveda are integral part of each other and harmonize each other. The concepts of Yoga Darshana are selectively integrated in Ayurveda to suit its objectives viz., ‘Swasthya Rakshana‟ (maintaining the...
ASSOCIATION OF PROSTATE SPECIFIC ANTIGEN LEVELS WITH POSITIVE BONE SCANS
Introduction: Prostate cancer has become a common problem among men around the world. This study was conducted to determine the clinical profiles of patients for whose bone scanning could be eliminated due to a low proba...
EVALUATION OF TRACE ELEMENTS IN KSHARA (ACHYRANTHES ASPERA).
Mineral elements have very important functions as they serve as structural components of tissues and also functions in cellular and basal metabolism. The plant Achyranthes aspera. is known for various medicinal propertie...
GASTRO-INTESTINAL EFFECT OF SHATAVARI GHRITA – A LITERARY REVIEW
Shatavari Ghrita is one of the herbal medicines containing Shatavari (Asparagus racemosus) root, milk and ghee. This has been described for treatment of various gastro intestinal disorders according to Ayurveda classical...
COMPARISON OF MENTAL HEALTH IN PEOPLE WITH AND WITHOUT PEPTIC ULCER DISEASE
Introduction: In many studies, connection between psychological factors and peptic ulcer disease considered but isn’t consensus about this issue. This study was carried out aimed to compare the mental health in people wi...