Sapium ellipticum (Hochst) Pax ethanol leaf extract modulates glucokinase and glucose-6–phosphatase activities in streptozotocin induced diabetic rats
Journal Title: Journal of Coastal Life Medicine - Year 2017, Vol 5, Issue 4
Abstract
Objective: To examine the effects of Sapium ellipticum (S. ellipticum) extract on hepatic activities of glucokinase and glucose-6-phosphatase in streptozotocin (STZ)-induced diabetic Wistar rats. Methods: STZ-induced diabetic Wistar rats (four groups, n = 8) were used in this study. S. ellipticum was assessed at two different doses, 400 and 800 mg/kg BW, in comparison with metformin (12 mg/kg BW) as a standard antidiabetic drug. All treatments were done orally (p.o), twice daily at 8 h interval for a period of 21 days. Glucose-6-phosphatase and glucokinase activities were respectively determined. Hepatic and muscle glycogen contents were estimated as well. Results: STZ caused significant decrease in glucose-6-phosphatase activity and concomitant increase in glucokinase activity. S. ellipticum especially at 400 mg/kg significantly restored glucokinase activity by 40.31% and glucose-6-phosphatase activity by 37.29%. These effects were though significantly lower than that of metformin which enhanced glucokinase activity by 94.76% and simultaneously inhibited glucose-6-phosphatase activity by 49.15%. S. ellipticum also improved hepatic glycogen level by 32.37% and 27.06% at 400 and 800 mg/kg, respectively. HPLC-MS analysis of active S. ellipticum fractions in dynamic MRM mode (using the optimized compound-specific parameters) revealed among other active compounds, the presence of amentoflavone, which has been associated with anti-diabetic function. Conclusions: The ability of S. ellipticum extract to concurrently inhibit glucose-6-phosphatase and activate glucokinase in this study suggests that it may be a treatment option for type 2 diabetes patients and the presence of amentoflavone in the plant extract may account for its anti-diabetic potential.
Phytochemical analysis, in vitro antioxidant and antibacterial activities of root extracts of Carduus macracanthus
Objective: To investigate phytochemical constituents, antibacterial and antioxidant activity of the root extracts of Carduus macracanthus (C. macracanthus). Methods: Phytochemical screening tests were conducted as per of...
Phytochemical constituents and larvicidal activity of Tragia involucrata Linn. (Euphorbiacea) leaf extracts against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae)
Objective: To determine the phytochemical and larvicidal activity of Tragia involucrata (T. involucrata) leaf extracts against fourth instar larvae of Ae. aegypti. Methods: Phytochemical analysis of hexane leaf extract w...
Toxicity of lemon grass Cymbopogon citratus powder and methanol extract against rice weevil Sitophilus oryzae (Coleoptera: Curculionidae)
Objective: To evaluate the toxicity potential of lemon grass [Cymbopogon citratus (C. citratus)] products against adult rice weevil, Sitophilus oryzae. Methods: Lemon grass (C. citratus) leaves were sundried for 7 days,...
Biotechnological leap in biopharmaceuticals: Current status and challenges ahead
Fermentation based vaccine production system is replaced by edible vaccine technology. Plant derived vaccines are produced by using transgenic plants which have immunogenic properties. For combating infectious diseases e...
Effect of ethanolic extract of Pericampylus glaucus (Lam) Merr on biochemical parameters in SpragueDawley rats with high fats diets
Objective: To evaluate the effect of ethanolic extract of Pericampylus glaucus (Lam) Merr (P. glaucus) on biochemical parameters in high fats diets treated rats. Methods: The effect of P. glaucus extract on biochemical p...