Sapium ellipticum (Hochst.) Pax Leaf Extract: In-vitro Antioxidant Activities and Lethal Dose (LD50) Determination in Wistar Rats
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 18, Issue 2
Abstract
Prior to this investigation, the LD50 and antioxidant activities of Sapium ellipticum leaf extract have not been reported, hence; the focus of this study. The LD50 of the extract was determined through three routes of administration using the method of Lorke. The Intraperitoneal (i.p) and intramuscular (i.m) LD50 values were determined as 979.80 and 1,341.60 mg/kg body weight (BW) respectively. Oral administration of the extract (to a dose of 45,000 mg/kg BW) did not cause any negative behavioral changes in the animals, and no mortality was recorded within and after 24h of the experiment. The antioxidant properties of the extract was assessed in vitro in terms of its free radical scavenging, metal chelating and reducing power activities as well as its ability to inhibit the formation of malondialdehyde (MDA), an index of lipid peroxidation. The total phenolic content of the extract was determined as 74.23±3.12 mg GAE/g. Data obtained indicate that the extract exhibited appreciable free radical scavenging activity (IC50 = 0.128 mgmL-1) and strong reducing power in comparison with butylated hydroxyl toluene (BHT, IC50 = 0.118 mgmL-1) and ascorbic acid (IC50 = 0.120 mgmL-1) used as reference antioxidants. The inhibition of linoleic acid induced-lipid peroxidation by the extract was comparable to that of BHT and greater than that elicited by ascorbic acid. In terms of metal chelating activity, ethylene diamine tetraacetic acid (EDTA) used as positive control elicited a significantly (p≤0.05) higher activity than the extract at all concentrations used. Findings from this study credit Sapium ellipticum ethanol leaf extract with significant antioxidant properties. The plant material may therefore be of immense relevance in combating oxidative stress and its related ailments.
Authors and Affiliations
O. M. Ighodaro, O. A. Akinloye
Keywords
Related Articles
- EP ID EP341030
- DOI 10.9734/BJMMR/2016/8253
- Views 47
- Downloads 0
Effect of Different Exposure Conditions and Developing Solution Concentration on the Clarity of Cervical Burnout in Bitewing Radiographs
Aims: Exposure and developing conditions can affect the occurrence of cervical burnout in bitewing radiographs which is one of the common false positive errors in these images. This study was designed to evaluate the eff...
Anemia Management Practice in French Hemodialysis Centers
Objective: Evaluate the hemoglobin (Hb) target values in hemodialysis (HD) patients treated with erythropoiesis-stimulating agents (ESA). Methods: Records of anemia parameters of HD patients treated during year 2012 in...
Grifola frondosa Extract Induced Acute Hepatic Injury
Aim: To describe a case of acute hepatic injury related to the use of Grifola frondosa in a patient with colon cancer. Case Presentation: Patient is a 67 year old female with stage IV poorly differentiated adenocarcinoma...
Calcium and Total Bilirubin Levels in Patients Co-Infected with HIV and Hepatitis C Viruses in Lautech Teaching Hospital, Osogbo, South West Nigeria
Aim: One third of HIV patients are co- infected with HCV. As HIV patients live longer this co-infection and its complications such as liver cirrhosis, hepatic carcinoma, metabolic syndrome are emerging as major manifesta...
Influence of Vitamin K Epoxide Reductase Complex 1 Gene Polymorphisms on Anticoagulation with Acenocoumarol in Patients with Cerebral Venous Thrombosis
Aims: Acenocoumarol, a commonly prescribed oral anticoagulant drug, exhibits wideinter-individual variability in response. This study aimed at evaluating the contribution of genetic variations in Vitamin K epoxide reduct...