Scale up Pseudomonas fluorescens-based biofertilizers production
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 4
Abstract
In previous studies, a semi-synthetic culture medium was optimized for the industrial production of a plant growth-promoting strain of Pseudomonas fluorescens. This work was proposed to develop the scale up for producing the biofertilizer. The criteria for scale up were selected taking into account the geometric non-similarity of the fermenters used (42 and 500 liters). The technical effectiveness of the resulting bioproduct was evaluated in liquid formulation on in vitro plants of banana cv. Manzano (Musa AAA) in the acclimatization stage (root immersion 10, 15, 20 and 25 min) and in the field cultivation of Ipomoea batatas applied to the soil at a rate of 20 l ha-1. The results showed that under the conditions tested, bacterial growth was obtained with similar kinetics on two scales. The fermentations concluded 12 hours after the start of the culture. The banana in vitro plants responded better when the immersions in the biofertilizer occurred for 20 min. In the cultivation of sweet potato a positive response was obtained when it was combined with mineral fertilizers and a reduction of 25% of the recommended dose was achieved. The production of a biofertilizer based on Pseudomonas fluorescens can be carried out in a 500 l stirred tank fermenter (effective volume 350 l) for 12 h with stirring speed of 266 rpm and air flow of 105 l min-1 which guarantees a concentration of 1x109 CFU ml-1 and its plant growth promotion effects.
Authors and Affiliations
María Cristina Pérez Peñaranda, Julio Oramas García, Emilio Arcadio Sotolongo Valdés, Jessica Valdivia Pérez, Yoandra Román Tabio, Yoel Beovides García, Jaime Simó González
Keywords
Related Articles
- EP ID EP697691
- DOI -
- Views 70
- Downloads 0
In silico identification of DELLA proteins in plants of Coffea arabica L. and Ananas comosus L. Merr
Gibberellins control various aspects of plants growing and development, including seed germination, stem elongation, leaves expansion, flower and seed development. They are also involved in phase changes in some species,...
Effect of immersion frequency on shoot multiplication of Bambusa vulgaris Schrader ex Wendland in RITA ® TIS
Bamboos are unique in bringing together a wide range of environmental services. The present work was conducted to determine the effect of immersion frequency of shoot multiplication of B. vulgaris grown in SIT. Three Im...
Conservation of potato mini tubers with the use of zeolite powdered
The application of natural products in seed conservation plays an important role in countries with tropical climates. With the objective of to determine the effect of the powdered zeolite in the conservation of potato...
Regeneration of plants via somatic embryogenesis in Phaseolus acutifolius A. Gray
Plant regeneration protocols are a requirement to develop plants transformation systems.The capacity of Phaseolus acutifolius A. Gray to regenerate plants from embryogenic callus formation was investigated. Two explants...
Designing a curriculum for Plant Biotechnology elective course for students in pre-university
To introduce the plant biotechnology in the curriculum of Vocational Pre-university Institutes of Sciences (IPVCE), increase the culture of the students in this specialty and motivate them to related careers, this stud...