Scale up Pseudomonas fluorescens-based biofertilizers production
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 4
Abstract
In previous studies, a semi-synthetic culture medium was optimized for the industrial production of a plant growth-promoting strain of Pseudomonas fluorescens. This work was proposed to develop the scale up for producing the biofertilizer. The criteria for scale up were selected taking into account the geometric non-similarity of the fermenters used (42 and 500 liters). The technical effectiveness of the resulting bioproduct was evaluated in liquid formulation on in vitro plants of banana cv. Manzano (Musa AAA) in the acclimatization stage (root immersion 10, 15, 20 and 25 min) and in the field cultivation of Ipomoea batatas applied to the soil at a rate of 20 l ha-1. The results showed that under the conditions tested, bacterial growth was obtained with similar kinetics on two scales. The fermentations concluded 12 hours after the start of the culture. The banana in vitro plants responded better when the immersions in the biofertilizer occurred for 20 min. In the cultivation of sweet potato a positive response was obtained when it was combined with mineral fertilizers and a reduction of 25% of the recommended dose was achieved. The production of a biofertilizer based on Pseudomonas fluorescens can be carried out in a 500 l stirred tank fermenter (effective volume 350 l) for 12 h with stirring speed of 266 rpm and air flow of 105 l min-1 which guarantees a concentration of 1x109 CFU ml-1 and its plant growth promotion effects.
Authors and Affiliations
María Cristina Pérez Peñaranda, Julio Oramas García, Emilio Arcadio Sotolongo Valdés, Jessica Valdivia Pérez, Yoandra Román Tabio, Yoel Beovides García, Jaime Simó González
Radiosensitivity to gamma rays ( 60 Co) in shoot tips of henequen
The current research was carried out with the objective of evaluating the radiosensitivity of shoot tips from henequen (Agave fourcroydes) to gamma radiations 60 Co and to determine the optimum dose of radiation for bree...
Establishment of a clonal bank of Caesalpinia spinosa (Mol.) O. Kuntz by selection of plus trees and grafting
In Ecuador, plant propagation techniques are not available commercially to establish clonal banks of Caesalpinia spinosa (Mol.) O. Kuntz (guarango) plus trees, which limits the development of in vitro propagation proto...
Effect of H 2 O 2 application during ‘Grande naine’-Mycosphaerella fijiensis interaction
In Musa spp. considerable economical lost are cause by Mycosphaerella fijiensis infection around the world, that is why the study of the pathosystem constitute a priority. However, the main mechanisms activated in bana...
In vitro multiplication of Paeonias sp. variety ‘SeSu’ in Temporary Immersion Systems
Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) a...
Use of Temporary Immersion Systems for the large-scale production of in vitro tubers of Solanum tuberosum L. var. Atlantic and study of its behavior in the field
A special temporary immersion system was designed in order to scale up the potato in vitro tubers production as an alternative method for potato seed production. An average of 2.6 in vitro tubers with an average fresh we...