Schwann cells culture with adipose stem cells and neuregulin to determine influencing factors applicable in nerve regeneration
Journal Title: Journal of allbiosolution - Year 2016, Vol 1, Issue 01
Abstract
Schwann cell (SC) cultures are required for neural regeneration, as for example in injured nerves. However, SC culture procedures need improvements. This study evaluates and compares growth of SC cultures without and with neuregulin, and in co-cultures with adipose mesenchymal stem cells (ASCs). Rat originated SCs and ASCs were cultured, maintained and subcultured to prepare monocultures and co-cultures for this study. Morphological, immunohistochemical and quantitative evaluations were performed at 48 and 96 h. In monocultures SCs were S100 and vimentin positive, appeared bipolar with small somata containing small and elongated nuclei, and tended to contact on their ends forming chains or clustering around other cell types. ASCs were CD 105 and vimentin positive, showed fibroblast-like shape with large rounded nuclei and distinct nucleoli, became larger along time and contacted with the neighboring cells. Continue reading
Authors and Affiliations
María Nelcy Orozco García1, Manuel J. Gayoso1, Sara Gayoso del Villar1, Girish K. Srivastava1, Manuel Garrosa1 1. Departamento de Biología Celular, Histología y Farmacología, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain.
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- EP ID EP680423
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Schwann cells culture with adipose stem cells and neuregulin to determine influencing factors applicable in nerve regeneration
Schwann cell (SC) cultures are required for neural regeneration, as for example in injured nerves. However, SC culture procedures need improvements. This study evaluates and compares growth of SC cultures without and wit...