Screening and Characterization of Exopolysaccharide Producing Mesophilic Lactobacillus acidophilus Isolated from Human Dental Caries
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 12
Abstract
Dental caries is an infectious, communicable disease that acid-forming bacteria of dental plaque can destroy tooth structure in the presence of fermentable carbohydrates such as sucrose, fructose, and glucose. It continuous to be a major problem in dentistry affecting the mankind even today. In this present investigation EPS producing cariogenic Lactobacillus acidophilus were isolated from various dental clinics were analyzed and characterized by phenotypic and 16SrDNA gene sequence for the production of exopolysaccharides. Further, the optimal conditions of temperature (30°C - 45°C) were determined. Lactobacillus acidophilus were identified and screened on the basis of the molecular weight of its plasmid DNA (100 kb). The EPS production by Lactobacillus acidophilus was confirmed by thin layer chromatography (TLC) analysis. Optimization of culture condition for mass EPS yield was studied. When the strains were grown in 10% (W/V) reconstituted with skim milk under different culture conditions. The result showed that culturing of Lactobacillus acidophilus strain - LACVG02, LACVG06, LACVG17, LACVG36, LACVG38, LACVG75, LACVG85 and LACVG92 at temperature (37°C) and pH (6.5) optimal for growth was also favourable for EPS production. Supplementation in 10% (w/v) skim milk with different concentration (1%,2%,3%,4% and 5%) of carbon sources (glucose, sucrose, maltose, dextrose and fructose) increased EPS production; glucose being more effective than other sugars. Supplementation with different concentration of nitrogen sources (Peptone, Beef, Yeast, Urease, Gelatin, Ammonium Ferric Citrate, Ammonium Molyptate, and Ammonium per Sulphate, Ammonium Acetate) at 1% - 5% (w/v) resulted in about two-fold increase in EPS production. The potentiality of Lactobacillus acidophilus in EPS production was assessed. EPS production by Lactobacillus acidophilus is partially growth associated. A maximum of 1.99 gm of EPS/ml was synthesized by LACVG06 in the skim milk medium supplemented with 5% (w/v) glucose and 5% (w/v) of different nitrogen sources increased in EPS production; ammonium acetate being more effective than other nitrogen sources by LACVG06 in skim milk production medium produce a maximum of 1.98 gm of EPS ∕ ml was synthesized at 37°C and at initial pH 6.5.
Authors and Affiliations
Vijayalakshmi. S, S. Rajasekar, A. Mohankumar
Keywords
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- EP ID EP250767
- DOI 10.9790/0853-1612055472
- Views 83
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