Screening and identification of soil bacteria for growth promotion of wheat (Triticum aestivum L.)
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 3
Abstract
Plant growth promoting rhizobacteria (PGPR) are well known to impact plant growth by various direct or indirect mechanisms. To investigate the effect of PGPR, a total of nineteen bacterial strains were isolated from wheat rhizospheric soil. These bacterial strains were characterized for their plant growth promoting (PGP) traits like indole-3-acetic acid (IAA) production, phosphate solubilzation, nifH gene amplification, catalase, oxidase, siderophore production, and gram staining. Identification of bacterial strains was done by using 16S rRNA gene sequencing and all bacterial strains were able to produced IAA and solubilize inorganic phosphate. The range for IAA and inorganic phosphate was between 2.81-107.39 μg mL-1 and 14.55 to 556.63 μg mL−1, respectively. Only two bacterial strains were successfully amplified for 400bp nifH gene and most of the isolated bacterial strains were positive for siderophore production test. On the basis of PGP activities nine strains were selected as most promising and tested for wheat growth under controlled chamber experiment. All tested PGPR significantly increased root length, shoot length, dry root weight, and dry shoot weight over control. Three strains i.e. (i) A18, (ii) A28 and (iii) A29 were selected on the basis of their performance under controlled chamber and further testing under pot and field conditions along with application of different doses of NP fertilizer. In pot and field experiments, significant increase was recorded in T6. These results support our hypothesis that use of PGPR or combinations of PGPR and chemical fertilizer can enhance the nutrient use efficiency of fertilizers and crop production.
Authors and Affiliations
Ummay Amara, Yong-Xia Wang, Xiao-Long Cui, Rabia Khalid, Safdar Ali, Ghulam Shabbir, Rifat Hayat
Keywords
Related Articles
- EP ID EP38613
- DOI -
- Views 167
- Downloads 0
Synthesis of new superabsorbent hydrogel based on lignin
Lignin as a polysaccharide has been chemically modified by graft copolymerization of 2- hydroxyethylmethacrylate (HEMA) an aqueous medium using ammonium persulfate (APS) as an initiator under argon atmosphere. Evidence...
Identification key to species of sphecini (Hymenoptera: Sphecidae: Sphecinae) in Iraq
In this study, 150 specimens of the Sphecini tribe (Hymenoptera: Sphecidae: Sphecinae), collected from different region of Iraq are investigated. Nine species belonging to two genera were determined; this species are: S...
Faecal coliforms and faecal streptococci community in the water of SidiChahed dam and these emissaries Mikkes and Mellah(Morocco): The importance of some environmental chemical factors
A bacteriological and chemical study was carried out within 1 year, from December 2012 to November 2013, on sidiChahed dam and these emissaries Mikkes and Mellah upstream of the SidiChahed dam of Morocco. It assessed t...
Evaluation of yield and yield components in promising genotypes of bread wheat in Ardabil region
Cereal has historically used as feed by man and today, various products prepare from cereals. The experiment was conducted in order to evaluate 10 promising genotypes of bread wheat in a randomized complete block desig...
Effects of different distillation methods on essential oil content and composition of Lippiacitriodora H.B.K.
The world production and consumption of essential oils and perfumes are increasing very fast. Production technology is an essential element to improve the overall yield and quality of essential oil. Hydro-distillation (...