Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2018, Vol 15, Issue 1
Abstract
Objectives: The aim of the study was to investigate the effects of methanol extracts from the flowers and leaves of Diplotaxis tenuifolia and Reseda lutea on the activity of AR, CAT, GST, and GPx. Materials and Methods: Total phenolic and flavonoid contents of the plant samples were evaluated using Folin-Ciocalteu reagent and aluminum chloride colorimetric methods. Also, the effects of extracts on CAT, GST, GPx, and AR enzyme activities were investigated using kinetic assays. Results: The highest phenolic and flavonoid contents were detected in the methanol extract of D. tenuifolia leaves with 144.49±0.29 mg gallic acid equivalent/L and 250.485±0.002 quercetin equivalent/L, respectively. The best activity profile for GST and GPx were observed in the extract of leaves belonging to D. tenuifolia with IC50 values of 121±0.05 and 140±0.001 ng/mL, respectively. According to the results, methanol extracts from leaves of R. lutea and D. tenuifolia showed no significant activity potential on AR. Moreover, none of the studied extracts demonstrated any reasonable CAT activation potential. Conclusion: The results indicated that leaves of D. tenuifolia had good effect on the antioxidant enzymatic defense system, which it makes it a good constituent of the daily diet.
Authors and Affiliations
Khalid Sharro ABDALRAHMAN, Merve Gülşah GÜNEŞ, Naznoosh SHOMALI, Belgin Sultan İŞGÖR, Özlem YILDIRIM
Keywords
Related Articles
- EP ID EP382432
- DOI 10.4274/tjps.82473
- Views 104
- Downloads 0
Simultaneous Estimation of Saxagliptin and Dapagliflozin in Human Plasma by Validated High Performance Liquid Chromatography - Ultraviolet Method
Objectives: The fixed dose combination of saxagliptin and dapagliflozin is a recently approved antidiabetic medication. It is marketed under the brand name Qtern. The aim of this study was to develop a simple, rapid, sen...
The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines
Objectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This...
Phytochemical Analysis and Antidepressant Activity of Ixora coccinea Extracts in Experimental Models of Depression in Mice
Objectives: The present study aims to assess the antidepressant activity of Ixora coccinea extracts in mice and phytochemical analysis of the active extract by GC-MS. Materials and Methods: After oral administration of...
The Apoptotic and Anti-apoptotic Effects of Pendimethalin and Trifluralin on A549 Cells In Vitro
Objectives: Pendimethalin and trifluralin are commonly used in many countries to control broadleaf weeds and grassy weed species because of their inhibitor effects on growth and cell division. In this study, we examined...
Characterization of Gamma-Irradiated Rosmarinus officinalis L. (Rosemary)
Objectives: Rosmarinus officinalis L., a member of the family Lamiaceae, is regarded as the spice with the highest antioxidant activity. Materials and Methods: In this study, the transmission electron microscopy, X-ray d...