Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2018, Vol 15, Issue 1
Abstract
Objectives: The aim of the study was to investigate the effects of methanol extracts from the flowers and leaves of Diplotaxis tenuifolia and Reseda lutea on the activity of AR, CAT, GST, and GPx. Materials and Methods: Total phenolic and flavonoid contents of the plant samples were evaluated using Folin-Ciocalteu reagent and aluminum chloride colorimetric methods. Also, the effects of extracts on CAT, GST, GPx, and AR enzyme activities were investigated using kinetic assays. Results: The highest phenolic and flavonoid contents were detected in the methanol extract of D. tenuifolia leaves with 144.49±0.29 mg gallic acid equivalent/L and 250.485±0.002 quercetin equivalent/L, respectively. The best activity profile for GST and GPx were observed in the extract of leaves belonging to D. tenuifolia with IC50 values of 121±0.05 and 140±0.001 ng/mL, respectively. According to the results, methanol extracts from leaves of R. lutea and D. tenuifolia showed no significant activity potential on AR. Moreover, none of the studied extracts demonstrated any reasonable CAT activation potential. Conclusion: The results indicated that leaves of D. tenuifolia had good effect on the antioxidant enzymatic defense system, which it makes it a good constituent of the daily diet.
Authors and Affiliations
Khalid Sharro ABDALRAHMAN, Merve Gülşah GÜNEŞ, Naznoosh SHOMALI, Belgin Sultan İŞGÖR, Özlem YILDIRIM
Keywords
Related Articles
- EP ID EP382432
- DOI 10.4274/tjps.82473
- Views 100
- Downloads 0
Investigation of the Antimicrobial Susceptibility Profile, Virulence Genes, and Epidemiologic Relationship of Clinical Salmonella Isolates
Objectives: The objectives of this study were to investigate the epidemiologic relationship, prevalence of the beta-lactamase and virulence genes of clinical ampicillin-resistant Salmonella enterica. Materials and Metho...
Flavin Containing Monooxygenases and Metabolism of Xenobiotics
This review summarizes recent information concerning the pharmacological and toxicological significance of the flavin-containing monooxygenases (FMOs). FMOs are a family of microsomal enzymes involving in the oxygenation...
Phytochemical Screening and Toxicological Evaluation of Sargassum wightii Greville in Wistar Rats
Objectives: The present study was conducted to identify the phytoconstituents present in different extracts of Sargassum wightii and to assess the toxicity of its ethanol extract. Materials and Methods: Successive solven...
The Ameliorative Effects of Pycnogenol® on Liver Ischemia-Reperfusion Injury in Rats
Objectives: Pycnogenol® (PYC®), a standardized extract from the bark of Pinus maritima, consists of different phenolic compounds. PYC® has shown to have protective effects on chronic diseases such as diabetes, asthma, ca...
Development and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Application
Objectives: This study aimed to prepare and evaluate mucoadhesive nanoparticle formulations of voriconazole, an antifungal drug, for mucosal application. It was also aimed to develop and validate a HPLC method of voricon...