Screening for Glucokinase (GCK) Gene Mutation in Gestational Diabetes Women in Western Region of Saudi Arabia
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 13, Issue 8
Abstract
Aim: The study involved screening of three exons of glucokinase gene (7, 8 and 9) to detect mutations at posi¬tions 682A>G, p.T228A, 895G>C, p.G299R and 1148C>A, p.S383X, respectively in Saudi pregnant women with or without gestational diabetes. Study Design: Retrospective study. Place and Duration of Study: The Obstetrics and Gynecology clinic of King Abdul-Aziz Hospital in South Jeddah between May and October 2013. Methodology: A sample of 100 Saudi pregnant women classified as healthy (control, N = 50 (pregnant women without diabetes)) or with gestational diabetes (N = 50) was analyzed for mutations in the GCK gene. Blood samples were taken and DNA was extracted from whole blood. The target part of GCK gene was amplified by the polymerase chain reaction (PCR). The specific amplified regions were digested by restriction enzymes: BstUI for exon 7, HhaI for exon 8 and BfaI for exon 9, and electrophoresed on agarose gel. Mutations were screened by restriction fragment length polymorphism. Results: Fasting glucose levels were statistically higher in gestational diabetes women than controls (P-value = 0.00). The difference in Glucose Challenge Test (GCT) values between two groups shows significante p-value = 0.00. However, We observed that there was no significant correlation between any of the GCK mutations and gestational diabetes in Saudi population. Conclusion: The glucokinase gene mutations do not affect glucose metabolism in pregnant Saudi women.
Authors and Affiliations
Sabah M. Hassan, Archana P. Iyer, Fahad A. Al-Abbasi
The Association of Eating Attitudes between Metabolic Control and Quality of Life in Patients with Type 1 and Type 2 Diabetes Mellitus
Aims: The combination of an eating disorder and diabetes mellitus (DM) puts patients at high risk of mortality and morbidity. The purpose of this study was to determine the association of eating attitudes between metabol...
Evaluation of Polymorphisms rs762624 and rs3176336 of CDKN1A Gene and Risk of Colorectal Cancer
Aims: Progressive loss of cell cycle control is an important feature on the colorectal cancer. CDKN1A gene encoded p21 protein that’s one of the cyclin-dependent kinase inhibitors, plays a key role in regulating the cell...
Comparison of Various Phenotypic Methods for the Detection of Metallo-beta-lactamases in Pseudomonas aeruginosa
Aims: To evaluate the screening antibiotic and phenotypic test that can be used to confirm metallo-beta-lactamase (MBL) production in clinical isolates of Pseudomonas aeruginosa and to find out the prevalent MBL gene in...
Search for Escherichia coli O157:H7 and Other Enterohaemorrhagic Escherichia coli in Diarrhoeal Cases in Abuja, Nigeria
Aims: To detect the sporadic involvement of shiga toxin producing Escherichia coli (STEC) O157:H7 or any other enterohaemorrhagic E. coli in gastro-intestinal (diarrhoeal) infections. Study Design: The study is a cross s...
Comparison of Pressure-controlled Inverse Ratio Ventilation versus Pressure-controlled Ventilation in Laparoscopic Cholecystectomy with LMA
Aims: In comparison with volume-controlled ventilation (VCV), the pressure controlled ventilation (PCV) improves oxygenation and ventilation. PCV method decreases peak airway pressure in laparoscopic surgery. The aim of...