Screening for rhodanese producing Bacterium in freshly pressed Cassava effluents of a Cassava processing industry channeled to Odo-Oba Stream in Ogbomoso-Nigeria
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 3
Abstract
Rhodanese is a key enzyme that plays an important role in cyanide detoxification. The enzyme was extracted, purified and physico-chemically characterised from Bacillus licheniformis which demonstrated the highest efficacy compared to the seven isolates of bacteria of the cassava processing industry effluent morpholologically and biochemically characterised. Statistical analysis was performed using one-way ANOVA and values were considered significant at p<0.05. This study showed that the optimum growth temperature was 350C at a pH 9.0. The highest duration time for the synthesis of rhodanese was at 40 hours. Potassium cyanide (KCN) and casein were the best carbon and nitrogen sources. The enzyme has a specific activity of 10.99 RU/mg, with a purification fold of 4.38, a percentage yield of 15.96%. The apparent Km for KCN and Sodium thiosulphate (Na2S2O3) were determined to be 30.24mM and 24.93mM respectively while their Vmax were 5.40 RU /ml/min and 5.07 RU /ml/min respectively. The optimum pH and temperature were 8.0 and 50 0C respectively. The enzyme showed a high stability at 500C. The enzyme showed specificity at 6.78 RU/ml/min for Na2S2O3 while it was inhibited by other sulphur containing substrates namely 2-mercaptoethanol, ammonium persulphate, and sodium metabisulphite The enzyme activity was not inhibited by metal ions such as (K+, Mg2+, Ba2+, Ni2+, Sn2+ and Na+) at 1mM and 10mM and was not significant (p>0.05). Therefore, B. licheniformis have the potentials of reducing cyanide pollution thereby enhancing effective management of cassava mill effluent before eventual discharge into the environment and this may be developed into a more effective tool for bioremediation.
Authors and Affiliations
Adeola Folashade Ehigie, Gbadebo Emmanuel Adeleke, Wasiu Ayinde Oladiran, Leonard Ona Ehigie
Keywords
Related Articles
- EP ID EP632217
- DOI 10.31018/jans.v11i3.2055
- Views 87
- Downloads 0
Monitoring the status of Human-wildlife conflict and its impact on community based conservation in Bandhavgarh tiger reserve, Madhya Pradesh, India
The Bandhavgarh Tiger Reserve is one of the famous tiger reserve in India. The human wildlife conflict is on rise for few years. A total of 194 human casualties and 1960 livestock depredation were reported between 2001 t...
Phytochemical screening and evaluation of anti-microbial and anti-oxidant activity of Elettaria cardamom (Cardamom)
The present study deals with the phytochemical screening and evaluation of antibacterial and antioxidant activities from the crude methanol extract of the seeds of cardamom, Elettaria cardamom. Crude methanol extract was...
Ultrasonic method of Biodiesel production from Palm Kernel
Biodiesel is a clean burning alternative fuel derived from chemical reactors produced from palm kernel oil, is currently spreading like a wind dust in the air. It is considered as the fuel for the future without rise in...
Economic loss assessment on juvenile fish catch due to forced non-selectivity in a selective fishing gear, gillnet along Mumbai coast, India
A study on quantity and value of juvenile fish landings was carried out in the gillnet fishery of three selected landing centers along Mumbai coast viz., Versova, Cuff Parade and Mahim in India using Out board motors (OB...
Specific identification, biology and symptoms of whitefly species infesting sunflower in South India
Whitefly species related to sunflower was identified as Bemisia tabaci (Gennadius). Further the identified whitefly species was confirmed to be indigenous B. tabaci on molecular basis by using B-biotype specific SCARs an...