Screening of bacterial strains for pectate lyase production and detection of optimal growth conditions for enhanced enzyme activity
Journal Title: Journal of Applied and Natural Science - Year 2017, Vol 9, Issue 1
Abstract
In the present study, the pectatelyase production by fifty two bacterial strains isolated from ramie grown soils were studied and the strain RDSM01 showed maximum pectate lyase activity. According to sequence homology of Genbank, the strain RDSM01 was identified as Bacillus subtilis (Genbank Accession No. KX035109). Maximum pectate lyase activity of the strain was observed when 1.5% (v/v) inoculum was added to the growth medium and was incubated for 48 hours at 34-370C and at pH 7.0. The relative activity of the strain was 19% higher when apple pectin was used as carbon source compared to citrus pectin. Maximum enzyme production (149.1 – 153.4 IU/ml) was recorded when ammonium chloride or ammonium sulphate at 0.4% concentration was used as nitrogen source. Thus, B. subtilis strain RDSM01 possessing high pectate lyase activity may be effectively utilized for removal of gum from ramie fibre, which is primarily made of pectin and hemicellulose.
Authors and Affiliations
Monidipta Saha, Rajib S. Rana, Biswanath Adhikary, Sabyasachi Mitra
Keywords
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- EP ID EP286479
- DOI 10.31018/jans.v9i1.1198
- Views 55
- Downloads 0
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