Screening of Health Care Workers of Intensive Care Units for Detection of Methicillin Resistant Staphylococcus Aureus Carrier State in Basaveshwara Teaching and General Hospital, Gulbarga
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 6
Abstract
Introduction: Methicillin resistant Staphylococcus aureus is an important cause of nosocomial infection particularly in intensive care units. Objectives: To know the prevalence of methicillin resistant staphylococcus aureus carriers state among health care workers of intensive care units of Basaveshwara Hospital, Gulbarga. Materials and Methods: 160 health care workers sampling was done from finger tips, nasal cavity and throat of the health care workers engaged in these areas. Specimens were inoculated onto glucose broth, blood agar plate & mannitol salt agar. The suspected colonies were subjected to standard testing procedure for identification of staphylococcus aureus(confirmed by coagulase test). All coagulase positive isolates were subjected for methicillin sensitivity on Muller Hilton agar using oxacillin discs. Results: A total of 160 health care workers were screened. i.e. 160 swabs on finger tips and web spaces, 144 swabs from anterior nares and 60 swabs from throat were collected. Of the 160 swabs from finger tips 88(55%) yielded Staphylococcus aureus, of which 26(16.25%) were methicillin resistant. Among 144 nasal swabs, staphylococcus aureus was isolated in 120(83.33%), from which 52(36.11%) yielded methicillin resistant staphylococcus. In 30 throat swabs, 24(40%) samples were positive for staphylococcus aureus and among them 2(3%) was methicillin resistant. Conclusions: Our study revealed that health care workers were the potential colonizers of methicillin resistant Staphylococcus aureus. This study helped us to alert the authority to shift these workers to other areas and to advocate appropriate medication to eradicate their carrier state
Authors and Affiliations
Dr Asharani S
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