Screening of small peptides from various germinating seeds having antimicrobial activity
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 1
Abstract
Antibiotics administered against bacterial infections develop resistant strains which are an alarming trend that impacts great significance in human health perspective. Research indicates that antimicrobial peptides (AMP) can be used as potential alternatives to antibiotics, without inducing early antimicrobial resistance. Most of AMPs reported in the data base were of animal origin while very few were reported from plant sources. In light of this, 50 types of germinating seeds were systematically investigated for the presence of small peptides having AMPs. Selected seeds were germinated on brown sheet at different time intervals and proteins were extracted with both liquid nitrogen and phosphate buffer (PBS) treatments. Small peptides of <10kDa were obtained by 5kDa flow through and the same was confirmed by SDS-PAGE. Extracted small peptides were checked for antimicrobial activity against four clinical isolates i.e., Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Results indicate that Soya, Barley, Maize, Jowar and Wheat germinating seeds were found to have more antimicrobial peptides acting on gram positive and gram negative bacteria.
Authors and Affiliations
KAMALA GOLLA1&2,, SS. VUTUKURU, J. USHA RANI, P. MEGHANATH, CHAND PASHA1
Keywords
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