Secondary Metabolites Isolated from Dichloromethane Fraction of Rough Lemon Stem and Hepatoprotective Effect of Limonianin
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 16
Abstract
Aim: To investigate the dichloromethane fraction obtained from an aqueous methanol extract of green stems of Citrus jambhiri Lush. and evaluate the hepatoprotective effect of limonianin. Study Design: Isolation and identification of secondary metabolite and hepatoprotective activity of limonianin. Place and Duration of Study: Faculty of Pharmacy, Zagazig University and Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, between February 2012 and January 2014. Methodology: Psyllic acid, sinensetin, nobiletin, 6-demethoxytangeretin, limonianin, hesperetin, quercetin were isolated from the green stem of Citrus jambhiri. Their identity was unambiguously confirmed via different spectroscopic methods (UV, MS, 1H NMR, 13C NMR, H-COSY, HSQC and HMBC). The liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity (TAC) were used to evaluate the hepatoprotective effect of limonianin. Results: Limonianin exhibited an equal or even better hepatoprotective activity than the known and medicinally used flavonoid mixture silymarin on human hepatoma cells (HepG2) against D-galactosamine-induced hepatotoxicity. Limonianin at concentration 5 µM exhibited a reduction of ALT, AST and MDA with 46.50, 42.10 and 24.36%, respectively while SOD and TAC were elevated with 38.56 and 391.67%, respectively compared with D-galactosamine (D-GaIN) treated cells. At concentration of 10 µM, limonianin shows elevation of SOD and TAC with 74.56 and 791.67%, respectively compared with silymarin (63 and 766.67%, respectively). Conclusion: The findings of this study showed that limonianin could be used as ideal hepatoprotective agent.
Authors and Affiliations
Dalia Hamdan, Michael Wink, Assem El-Shazly
Keywords
Related Articles
- EP ID EP344560
- DOI 10.9734/BJPR/2014/12311
- Views 76
- Downloads 0
Mesoporous Silica as a Carrier for Amorphous Solid Dispersion
In the past decade, the discovery of active pharmaceutical substances with high therapeutic value but poor aqueous solubility has increased, thus making it challenging to formulate these compounds as oral dosage forms. T...
Antimicrobial, Antioxidant and Anticancer Activities of Turnera ulmifolia (Yellow Alder) Callus
Aim: The current work aims to study the effect of ethanolic and methanolic extracts of Turnera ulmifolia callus on antimicrobial, antioxidant and anticancer activities. Study Design: The Five grams of leaf derived 30 day...
Effect of Low Radiation Dose on Cisplatin Induced Hepato- Testicular Damage in Male Rats
Aim: The aim of this study was to evaluate the synergistic effect of low radiation dose with the chemotherapeutic drug in order to find possible way to lessen the harmful effects during chemo-radiotherapy. Study Design:...
Antibacterial and Antifungal Properties of Some Wild Nutraceutical Plant Species from Nebbi District, Uganda
Aims: The purpose of this study was to investigate the antibacterial and antifungal properties of selected wild nutraceutical plants from Nebbi district in Uganda. Study Design: Experimental study. Place and Duration of...
Bioactive Secondary Metabolites from Terrestrial Streptomyces baarnensis MH4
Aims: Isolation, screening and identification of potent bioactive compounds producing Streptomyces sp. from the terrestrial soil of Egypt. Fermentation study, product recovery, purification and its characterization was a...