SENSITIVE AND RAPID HPLC METHOD FOR THE DETERMINATION OF PIOGLITAZONE IN RAT SERUM
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2011, Vol 3, Issue 1
Abstract
A sensitive, accurate and rapid high-performance liquid chromatography with UV-visible detection (HPLC-UV) method for the determination of Pioglitazone in rat serum has been developed. Rosiglitazone was used as internal standard. Pioglitazone and Rosiglitazone are extracted from serum using a liquid–liquid extraction procedure using ethyl acetate. Isocratic separation of Pioglitazone and Rosiglitazone is carried out using a reversed-phase phenomenex C18 (250 mm × 4.6 mm, 5µm) column with mobile phase consisting of methanol and 30 mM ammonium acetate buffer (pH adjusted to 5 with ortho-phosphoric acid) in the ratio 60:40 (v/v) and quantified by UV detection at 269 nm. Analytical run time was less than 10 min. Mean recovery was 97.12% for 0.1-10µg/ml concentrations. The assay exhibited good linear relationship. Quantification limit was at 50ng/ml of Pioglitazone and accuracy and precision were over the concentration range of 0.1-10µg/ml. This method can be used for routine clinical monitoring of Pioglitazone.
Authors and Affiliations
CH Ravikanth, A Anil Kumar, V Uday Kiran, S Prashanth, B Madhu, Y Narsimha Reddy
SUBSTITUTED THIAZOLE LINKED MURRAYANINE-SCHIFF’S BASE DERIVATIVES AS POTENTIAL ANTI-BREAST CANCER CANDIDATES: FUTURE EGFR KINASE INHIBITORS
Cancer is the second leading causes of mortality across the planet which has had affected millions. In spite of massive efforts in producing new molecules and chemotherapeutic approaches for managing cancer, it continued...
QUANTITATIVE DETERMINATION OF MIFEPRISTONE IN PHARMACEUTICAL SAMPLES BY, VISIBLE SPECTROPHOTOMETRIC METHOD USING CE (IV) AS AN ANALYTICAL REAGENT
A simple, sensitive and accurate visible spectrophotometric method has been developed for the assay of mifepristone. The method is based on the colour reaction between Ce (IV) and mifepristone in the pH range 1.0-5.0.The...
DESIGN AND EVALUATION OF COLON TARGETED DOSAGE FORM CONTAINING MESALAZINE USING PH DEPENDENT POLYMERS
Colonic drug delivery has gained importance not just for the delivery of the drugs for the treatment of local diseases associated with the colon like Crohn’s disease, ulcerative colitis and irritable bowel syndrome but a...
THE RELATIONSHIP BETWEEN MAIN POLYPHENOL COMPONENTS AND FREE RADICAL SCAVENGING ACTIVITY OF SELECTED MEDICINAL PLANTS
This study investigates the relationships between the main polyphenols and DPPH radical scavenging activity (RSA) in extracts from some of the most common wild and cultivated species from the Achillea millefolium group,...
EVALUATION OF EX VIVO THROMBOLYTIC ACTIVITY AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THESPESIA POPULNEA LEAF EXTRACT
Thrombotic disorders like myocardial and cerebral infarction are fatal blood clotting related diseases. Synthetic therapeutics used in such disorders has serious adverse effects, so there is a need to investigate some mo...