SENSITIVITY COMPARISON OF ELISA AND RAPID SCREENING TECHNIQUES FOR THE DETECTION OF HBsAg AMONG CHRONIC LIVER DISEASE (CLD) PATIENTS IN A TERTIARY CARE HOSPITAL, SOUTH BIHAR, INDIA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 71
Abstract
BACKGROUND Hepatitis B is a major health problem in India. Based on the prevalence of hepatitis B carrier in India is the intermediate endemic level of hepatitis B surface antigen (HBsAg). This study aims to determine the frequency of HBV by screening of chronic liver disease (CLD) patients. This study also aims to compare the sensitivity between two diagnostic tests; one step rapid test strip device (RIA) and Enzyme Linked Immunosorbent Assay (ELISA). MATERIALS AND METHODS A cross-sectional study was carried out in adult patients with liver disease attending the Hepatology OPD, Tertiary Care Hospital in Nalanda (Bihar), India. Age, gender and clinical history of the patients were recorded. Blood specimen was screened for Hepatitis B surface Antigen (HBsAg) using one step rapid test strip device and Enzyme Linked Immunosorbent Assay (ELISA). RESULTS Five hundred patients were enrolled in the study. The mean age of infected patients in the study group was 37.7 ± 1.32 years (range 19 to 76 years). Of the 500 samples tested, 18.4% were positive for HBsAg by HEPA-SCAN HBsAg ELISA Test and 13.4% were positive by Hepacard one step rapid test. Considering the results of HEPA-SCAN HBsAg ELISA Test, the sensitivity, specificity, PPV and NPV of ELISA were 97.87%, 99.75%, 98.92% and 99.51% respectively. The sensitivity, specificity, PPV and NPV of Hepacard one step rapid test were 97.10%, 99.76 %, 98.52% and 99.54% respectively. CONCLUSION This study shows that frequency of HBV is high in India and the incidence is greater in males than the females. We also noted that in comparing both methods (ELISA method and the rapid test strip) for assessing the presence of HBsAg, ELISA test method was found to be more sensitive than the rapid test strip device. Therefore, we recommend strongly, that ELISA method be used to confirm test results obtained from the one step rapid test, when screening for chronic liver disease (CLD).
Authors and Affiliations
Imtiaz Ahmad, Asim Mishra, Chandan Kumar Poddar, Pawan Kumar Chaudhary, Maheshwar Narayan Singh
Keywords
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- EP ID EP265520
- DOI 10.14260/Jemds/2017/1097
- Views 82
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