SENSITIVITY COMPARISON OF ELISA AND RAPID SCREENING TECHNIQUES FOR THE DETECTION OF HBsAg AMONG CHRONIC LIVER DISEASE (CLD) PATIENTS IN A TERTIARY CARE HOSPITAL, SOUTH BIHAR, INDIA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 71
Abstract
BACKGROUND Hepatitis B is a major health problem in India. Based on the prevalence of hepatitis B carrier in India is the intermediate endemic level of hepatitis B surface antigen (HBsAg). This study aims to determine the frequency of HBV by screening of chronic liver disease (CLD) patients. This study also aims to compare the sensitivity between two diagnostic tests; one step rapid test strip device (RIA) and Enzyme Linked Immunosorbent Assay (ELISA). MATERIALS AND METHODS A cross-sectional study was carried out in adult patients with liver disease attending the Hepatology OPD, Tertiary Care Hospital in Nalanda (Bihar), India. Age, gender and clinical history of the patients were recorded. Blood specimen was screened for Hepatitis B surface Antigen (HBsAg) using one step rapid test strip device and Enzyme Linked Immunosorbent Assay (ELISA). RESULTS Five hundred patients were enrolled in the study. The mean age of infected patients in the study group was 37.7 ± 1.32 years (range 19 to 76 years). Of the 500 samples tested, 18.4% were positive for HBsAg by HEPA-SCAN HBsAg ELISA Test and 13.4% were positive by Hepacard one step rapid test. Considering the results of HEPA-SCAN HBsAg ELISA Test, the sensitivity, specificity, PPV and NPV of ELISA were 97.87%, 99.75%, 98.92% and 99.51% respectively. The sensitivity, specificity, PPV and NPV of Hepacard one step rapid test were 97.10%, 99.76 %, 98.52% and 99.54% respectively. CONCLUSION This study shows that frequency of HBV is high in India and the incidence is greater in males than the females. We also noted that in comparing both methods (ELISA method and the rapid test strip) for assessing the presence of HBsAg, ELISA test method was found to be more sensitive than the rapid test strip device. Therefore, we recommend strongly, that ELISA method be used to confirm test results obtained from the one step rapid test, when screening for chronic liver disease (CLD).
Authors and Affiliations
Imtiaz Ahmad, Asim Mishra, Chandan Kumar Poddar, Pawan Kumar Chaudhary, Maheshwar Narayan Singh
Keywords
Related Articles
- EP ID EP265520
- DOI 10.14260/Jemds/2017/1097
- Views 58
- Downloads 0
TARGET SCORE OF RIQAS AND SIGMA METRICS FOR EVALUATING THE ANALYTICAL PERFORMANCE OF THYROID FUNCTION TESTING ON ADVIA CENTAUR XPT IMMUNOASSAY ANALYSER
BACKGROUND Quality test reporting is essential part of diagnosis in any aliment, especially where the symptoms and signs are subtle like in thyroid disease. Quality assurance in clinical laboratory is presently done by b...
TO STUDY THE COMPARISON OF ORAL IRON VERSUS PARENTERAL IRON SUCROSE IN THE TREATMENT OF POSTPARTUM ANAEMIA
BACKGROUND Anaemia is an important indirect cause of maternal mortality and morbidity in third world countries and contributes to 20% maternal deaths (WHO). 27% women in the postpartum period were found to be anaemic wit...
CLINICAL STUDY OF NECK SWELLINGS
BACKGROUND Neck swelling is a common presentation in the course of number of diseases. This condition is generally not a disease by itself, rather it may be a symptom of many possible underlying problems. MATERIALS AND M...
STUDY OF PREVALENCE AND RISK FACTORS OF BRONCHIAL ASTHMA IN SCHOOL CHILDREN IN URBAN AND RURAL AREAS OF KAKINADA
In India the prevalence of asthma has increased over the last two decades, especially in children. There is scarcity of data on prevalence of asthma in school children. The aim of the study was to estimate the pr...
CLINICOPATHOLOGICAL STUDY OF NASAL MASS- A TERTIARY CARE HOSPITAL BASED STUDY
BACKGROUND The nose is the most prominent feature of the face and often catches the attention of the onlookers whenever it is deformed or altered in shape. Frequently patients attend ENT clinic with complaints of nasal o...