SENSITIVITY TO ANTIBIOTICS OF PLANKTON AND BIOFILM CULTURE OF STAPHYLOCOCCUS EPIDERMIDIS
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 1
Abstract
The aim was to conduct the comparative analysis of the sensitivity of 20 S. epidermidis strains of plankton and biofilm to antibiotics. Methods. Identification of clinical strains of staphylococci was performed using morphological, physiological and biochemical characteristics. Sensitivity to 19 antibiotics was determined using the disc-diffusion method. The study tested the ability for film formation in 96-well plate immunoassay. Results. There were established that the sensitivity of planktonic and biofilm forms clinical isolates of S. epidermidis to antibiotics was significantly different: MIC for ofloxacin was 0.15–3.0 μg/ml (planktonic form) and 0.3–5.0 μg/ml (biofilm form); for levofloxacin respectively 0.3–1.5 μg/ml and 0.8–3.0 μg/ml; for doxycycline hydrochloride 0.5–2.0 μg/ml and 1.2–4.5 μg/ml; for ceftriaxone 8.0–15.0 μg/ml and 15.0–30.0 μg/ml; for gentamicin 4.0–15.0 μg/ml and 14.0–30.0 μg/ml. Conclusion. It is determined that MIC antibiotics that inhibit the growth of plankton cultures on average are 2–5 times less compared to MIC, inhibiting biofilm formation.
Authors and Affiliations
O. I. Sidashenko, O. S. Voronkova, T. M. Shevchenko, O. A. Sirokvasha
Keywords
Related Articles
- EP ID EP395251
- DOI 10.18524/2307-4663.2014.1(25).48205
- Views 97
- Downloads 0
THE CREATION AND THE ASSESSMENT OF THE BIOTECHNOLOGICAL VALUE OF THE LACTOBACILLI COMPOSITION
Aim. To determine the biotechnological potential of lactobacilli isolated from raw meat and collection strains and the compositions which were created on their bases. Methods. Lactobacilli isolated from the meat samples...
BIOFILM FORMATION AND MOTILITY OF BACTERIA PSEUDOMONAS AERUGINOSA WITH DIFFERENT C-DI- GMPLEVEL
Aim: Biofilm formation abilities determination in Pseudomonasaeruginosa strains with different cyclic dimeric guanosine monophosphate (c-di-GMP) level and swimming, swarming, and twitching motilities. Materials and metho...
MYCOLYTIC ACTIVITY OF PHOSPHATE MOBILIZING SOILBA CTERIA OF GENUS BACILLUS COHN
The aim of this research was study of the chitinolytic activity of phosphateobilizing soil bacteria of genus Bacillus and their antagonistic properties towards microscopic fungi. Methods. In this research 16 strains of p...
THREONINE MUTANT STRAIN-PRODUCER BREVIBACTERIUM FLAVUM ІМВ В-7446 CHARACTERISTICS AND OPTIMIZATION PROCESS OF ITS BIOSYNTHESIS
Aim. Identification and optimization process of biosynthesis threonine of mutant strain-producer Brevibacterium flavum ІМВ В-7446. Methods. The strain Brevibacterium flavum ІМВ В-7446 was studied using standard microbiol...
METAGENOME 16S RRNA GENE ANALYSIS OF THE BLACK SEA MICROBIAL DIVERSITY IN THE REGION OF THE ZMIINIY ISLAND
The aim of the study was to determine the marine microbial biodiversity of the Zmiiniy island coastal seawater with the help of metagenomic analysis. Methods. The dualindexing primers were used to amplify v4 region of th...