SENSITIVITY TO ANTIBIOTICS OF PLANKTON AND BIOFILM CULTURE OF STAPHYLOCOCCUS EPIDERMIDIS
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 1
Abstract
The aim was to conduct the comparative analysis of the sensitivity of 20 S. epidermidis strains of plankton and biofilm to antibiotics. Methods. Identification of clinical strains of staphylococci was performed using morphological, physiological and biochemical characteristics. Sensitivity to 19 antibiotics was determined using the disc-diffusion method. The study tested the ability for film formation in 96-well plate immunoassay. Results. There were established that the sensitivity of planktonic and biofilm forms clinical isolates of S. epidermidis to antibiotics was significantly different: MIC for ofloxacin was 0.15–3.0 μg/ml (planktonic form) and 0.3–5.0 μg/ml (biofilm form); for levofloxacin respectively 0.3–1.5 μg/ml and 0.8–3.0 μg/ml; for doxycycline hydrochloride 0.5–2.0 μg/ml and 1.2–4.5 μg/ml; for ceftriaxone 8.0–15.0 μg/ml and 15.0–30.0 μg/ml; for gentamicin 4.0–15.0 μg/ml and 14.0–30.0 μg/ml. Conclusion. It is determined that MIC antibiotics that inhibit the growth of plankton cultures on average are 2–5 times less compared to MIC, inhibiting biofilm formation.
Authors and Affiliations
O. I. Sidashenko, O. S. Voronkova, T. M. Shevchenko, O. A. Sirokvasha
Keywords
Related Articles
- EP ID EP395251
- DOI 10.18524/2307-4663.2014.1(25).48205
- Views 106
- Downloads 0
FORMATION OF THE MULTIPLE GRAPE SHOOTS IN CULTURE IN VITRO ON DIFFERENT NOURISHING MEDIA
Semi-liquid nutrient media were used for the multiple grape shoots induction. Aim of the research was to study the agar concentration effect in different nutrient media and define influence of the media consistency on th...
MONITORING OF SPECIES COMPOSITION OF FUNGI OF THE GENUS FUSARIUM IN SEED MATERIALS OF WINTER WHEAT ON UKRAINIAN TERRITORY
Aim. To investigate strain composition of fungi of the genus Fusarium in winter wheat grain in Ukraine. Methods. Contamination of wheat grains with fungi was detected during their sprouting in rolls of filter paper. Mole...
CHARACTERISTIC FEATURES OF КЕY-LIKE BACTERIOPHAGES OF ERWINIA AMYLOVORA
Aim. To study the heterogeneity of phage isolates obtained from twigs and leaves of pear affected with Erwinia amylovora. Methods. The heterogeneity of phage isolates was studied using the system included low pressure li...
RESISTANCE OF LACTOBACILLI STRAINS ISOLATED FROM DIFFERENT SOURCE TO SOME AGGRESSIVE FACTORS OF THE DIGITAL TRACT
Aim. To study the resistance of lactobacilli strains to some aggressive factors of gastrointestinal tract in vitro depending on the primary isolation source. Methods. 13 lactobacilli strains isolated from self-fermenting...
ADENOVIRUS INFECTION AS ONE OF POSSIBLE CAUSES OF FATTY HEPATOSIS IN ONE DAY–OLD CHICKENS
Aim. To investigate the role of adenovirus infection in the development of fatty liver disease in one day-old chicks. Methods. The condition of the internal organs of poultry assessed by autopsy, the amount of vitamin A...