Separating,screening and identification of an antimicrobial endophytic bacterium from Ficus microphylla L.f.
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 2
Abstract
"This study was done to screen and identify the endophytes with antibacterial activity from Ficus microcarpa L.f. in order to develop biocontrol agents. Endophytes were isolated by tissue block method and tissue homogenate method. The bacteriostatic activity of endophytic bacteria was determined by cup dish method and colony growth rate method. Identifing the endophytic bacteria through the strain characteristics and bacteria morphology observation,physiological and biochemical characteristic test,determination of 16S rDNA gene sequences,and construction of phylogenetic tree. The results showed that one of the endophytes named QYPT-B01 had antibacterial activity. The fermentation filtrate of strain QYPT-B01 had strong bacteriostatic effect on Pseudomonas aeruginosa, Escherichia coli and Aspergillus niger. The composition of active substances in fermentation broth and its antagonistic effect on plant pathogenic bacteria could be further studied in the future."
Authors and Affiliations
Xue-ping DENG
Study on resistance of banana fusarium wilt detected by using crude toxin in vitro culture
In the process of banana tissue culture, the crude toxin of Fusarium oxysporum f. sp. cubense was added to the tissue culture medium to determine the sensitivity of different varieties of banana towards the crude toxin,...
Effects of different nitrogen concentrations on growth and chemical composition of a freshwater microalga Scenedesmus obliqnus
An experiment was carried out to evaluate the effects of different nitrogen concentrations on growth and chemical composition of a freshwater microalga Scenedesmus obliqnus. In this study,the culture solutions were desi...
Response of maize yields variability under different fertilizers deficiency scenarios to ecological factors in mountainous region
"Based on the records of Soil Test & Formulated Fertilization Project implemented during 2008 to 2009 in Chongqing,combining climatic data during the maize growth period and terrain variables,the response of maize yiel...
Analysis and enlightenment of sales characteristics of fresh litchi on E-commerce platform
The development of litchi e-commerce has a profound influence on the development of litchi industry. It is of great significance to understand the characteristics of the marketing of litchi ecommerce, to optimize the lit...
Optimization of process parameters under solid state fermentation using Eucommiae cortex as medicinal substrate by Cordyceps militaris( L.) Link
"The yields of pinoresinol diglucoside( PDG) and cordycepin in the fermentation products using Eucommiae cortex as medicinal substrate by Cordyceps militaris( L.) Link. were improved,and optimal conditions for ferme...