SEPARATION AND ANALYSIS OF AMLODIPINE/BENAZEPRIL COMBINATION IN CAPSULES BY A NOVEL ION PAIR LIQUID CHROMATOGRAPHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 1
Abstract
Objective: The objective of this study was to develop and validate a novel ion-pair liquid chromatography method, in order to separate and assay of amlodipine/benazepril combination in capsules. This method was a fast, practical and additional choice in quality control laboratories. Methods: The chromatographic conditions comprised of a classical C18-type stationary phase (250 × 4.6 mm, 5μ), with a mobile phase consisting of: 45% of 10-3 M of cetrimide and 55% acetonitrile. The flow rate was 1 ml/min; the detection wavelength was at 242 nm, under ambient temperature. Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products and excipients. Conclusion: The results presented in this paper showed that the developed method was fast and applicable, for the separation and determination of amlodipine/benazepril combination in capsules.
Authors and Affiliations
Saleh Trefi, Yaser Bitar
Keywords
Related Articles
- EP ID EP566072
- DOI 10.22159/ijpps.2019v11i1.29672
- Views 74
- Downloads 0
ANTIHYPERGLYCEMIC AND ANTIDYSLIPIDEMIC POTENTIAL OF IPOMOEA BATATAS LEAVES IN VALIDATED DIABETIC ANIMAL MODELS
Objective: The present study was undertaken to investigate the antidiabetic potential of the leaves of Ipomoea batatas.Methods: The crude powder, 95% ethanolic, 50% ethanolic and aqueous extracts of Ipomoea batatas leave...
NEAR-INFRARED SPECTROSCOPY: A POTENTIAL NEW MEAN OF ASSESSING MULTICOMPONENT POLYHERBAL FORMULATION ON WAY BEFORE AND AFTER EXTRACTION
Objective:Traditional polyherbal medicine and their preparations have been widely used for the thousands of years in developing and developed countries owing to its natural origin and lesser side effects or dissatisfacti...
USE OF LACTIC ACID AND SPAN 80 IN THE FORMULATION OF LIPID BASED IMIQUIMOD VESICLES FOR GENITAL WARTS
Objective: The objective of present study was formulation development of imiquimod using lactic acid and span 80 for topical delivery to cure genital warts.Methods: Lipid based vesicles (LBV) of 2% imiquimod were prepare...
EVALUATION OF ANTIBACTERIAL AND HAEMOLYTIC ACTIVITY OF PHYTOCHEMICALS FROM FRESHWATER MICROALGA, EUGLENA VIRIDIS (EHREN)
Objective: The main aim of this research work was to evaluate the antibacterial and haemolytic activities of different extracts of Euglena viridis (E. viridis), a freshwater microalga.Methods: The solvent extraction has...
QUERCETIN, ENCAPSULATED QUERCETIN AND ITS APPLICATION- A REVIEW
Flavonoids are plant secondary metabolite shows a wide range of pharmacological and biological functions. Among the flavonoids, quercetin gained special attention for its potential therapeutic activities. The aim of this...