SEPARATION AND DETERMINATION OF THE S-ISOMER OF (10-CAMPHORSULFONYL) OXAZIRIDINE IN A BULK DRUG SUBSTANCE BY NORMAL-PHASE LIQUID CHROMATOGRAPHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 7
Abstract
Objective: To develop novel, simple and accurate enantiomeric separation of (10-Camphorsulfonyl) oxaziridine by normal-phase high-performance liquid chromatographic method as per ICH guidelines.Methods: The S-isomer and R-isomer of (10-Camphorsulfonyl) oxaziridine were baseline resolved on a Chiralcel OD-H (250 x 4.0 mm i. d, 5 mm) column using a mobile phase system containing n-Hexane: ethanol: trifluoroacetic acid (90:10:0.1 v/v/v.) at detector wavelength 210 nm and column temperature 30 °C. The chromatographic resolutions between S-isomer and R-isomer were found three. The developed method was extensively validated according to ICH guidelines.Results: Good linearity was observed for S-isomer over the concentration range of 900–9000 ng/ml, with the linear regression (Correlation coefficient R = 0.999) and proved to be robust. The limit of detection and limit of quantification of S-isomer was found to be 400 and 900 ng/ml, respectively for 20 ml injection volume. The percentage recovery of S-isomer was ranged from 97.0 to 102.0 in bulk drug samples of (10-Camphorsulfonyl) oxaziridine. (10-Camphorsulfonyl) oxaziridine sample solution and mobile, phase was found to be stable for at least 48 hours. The proposed method was found to be suitable and accurate for the quantitative determination of S-isomer in bulk drugs.Conclusion: A novel, simple and accurate normal phase LC method was described for the enantiomeric separation of 10-Camphorsulfonyl Oxaziridine is precise and specific.Â
Authors and Affiliations
Hemsagar P. Jadhav, Dnyandeo B. Pathare
Keywords
Related Articles
- EP ID EP578519
- DOI -
- Views 70
- Downloads 0
BIOLOGICAL EVALUTION OF THIAZOLE DERIVATIVES BEARING DITHIOCARBAMATE MOIETY AS POTENTIAL CHOLINESTERASE INHIBITORS
Objective: This study aimed to synthesize some thiazole derivatives bearing dithiocarbamic acid esters and to evaluate their anticholinesterase activity.Methods: 2-Chloro-N-[4-(2-methyl-4-thiazolyl)phenyl]acetamide was s...
POLYMERIC MATERIALS AS PLATFORMS FOR TOPICAL DRUG DELIVERY: A REVIEW
With the emergence of novel and more effective drug therapies, increased importance is being placed upon the drug delivery technology. Topical formulations are attractive alternatives to oral formulations and offer sever...
A COMPARATIVE ANALYSIS OF VITAMIN-C CONCENTRATION IN COMMERCIAL FRUIT JUICES AND FRESH FRUITS OF NEPAL WITH EFFECT OF TEMPERATURE
Objective: It was aimed to determine the best storage temperature for fruits to achieve the highest quality of vitamin-C and to compare vitamin C concentration between commercial fruits juices and fresh fruit juices in b...
PRODUCTION AND PURIFICATION OF ANGIOTENSIN-CONVERTING ENZYME INHIBITOR BY SELECTED BACTERIAL STRAIN FOR CANCER THERAPY
Objective: The present study was planned to explore safer, innovative and economic Angiotensin-converting enzyme inhibitors (ACEi) from beef extract by the action of a proteolytic Micrococcus luteus. Cytotoxicity of the...
KINETICS AND MODELING OF TANNASE PRODUCTION USING ASPERGILLUS FOETIDUS IN BATCH FERMENTATION
Objective: To produce tannase enzyme using Aspergillus foetidus with red gram husk as substrate in batch fermentor and investigate the suitable un structured kinetic model for the system.Methods: The present study was do...