Serological and molecular characterization of recent lumpy skin disease virus isolates from naturally infected previously vaccinated cattle in Egypt
Journal Title: Journal of Veterinary Medical Research - Year 2018, Vol 25, Issue 1
Abstract
lumpy skin disease virus (LSDV) was isolated, from naturally infected cattle that have a history of previous vaccination with live attenuated sheep pox virus (SPV) vaccine. The virus was isolated on chorio-allantoic membranes (CAM) of embryonated chicken eggs (ECE) and Madin Darby Bovine Kidney Cells (MDBK) and identified by agar gel precipitation test (AGPT) and immunofluorescent antibody technique (IFA). Characteristic pock lesions and intracyptoplasmic flourescene granules are identified respectively. Molecular characterization using polymerase chain reaction (PCR) using specific primer for G-Protein Coupled Chemokine Receptor Gene of LSDV isolates specific amplified product 554 bp. Sequence analysis revealed tow new isolates of LSDV.
Authors and Affiliations
Tamam, S. M. , El-Shereif, N. M. , Shokier, K. A
Keywords
Related Articles
- EP ID EP658273
- DOI -
- Views 228
- Downloads 0
Comparative pharmacokinetic and renal clearance study of ceftiofur in cross breed Friesian and Buffalo calves
The pharmacokinetic profile of ceftiofur sodium, a third generation cephalosporin, was studied in both Friesian and buffalo calves following a single intravenous and intramuscular administration of 2.2 mg kg-1 b.wt. in a...
Monitoring the hygienic quality of underground water in different localities in Egypt and Libya
The present study was carried out in six provinces in both Egypt and Libya throughout the period from January to October 2014. These areas were (Beni-Suef, Ismailia, and Matrouh district) in Egypt and (Tripoli, Zliten, a...
Effects of chromium piclonate and ascorbic acid supplementation on growth performance, carcass traits, blood constituents and picture of growing kids under the summer conditions
The present work was performed to study the effect of chromium piclonate and ⁄ or ascorbic acid supplementation on the performance of growing kids during hot summer season (33.6oC and 74.2% RH). A total number of twenty...
Radiologic, Ultrasonic and pathological assessments of locally applied estrogen on promotion of experimentally induced tibial fracture healing in rats
The objective of the present study was to evaluate the ability of estrogen to promote induced tibial fracture healing in a rat model. The study was conducted on 14 albino rats divided into two equal groups (seven rats in...
Bacteriological studies on calves affected with respiratory manifestations
Bovine respiratory disease (BRD) is the most common and costly disease affecting beef cattle calves in the world. The objectives of this study were to put a high light on epidemiology of P. multocida and M. haemolytica a...