SEROSCREENING OF LACTATING CATTLE FOR COXIELLOSIS BY TRANS-PCR AND COMMERCIAL ELISA IN KERALA, INDIA
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
The sero-epidemiology of Coxiella burnetii infection in domestic ruminants, which are considered as prime reservoirs, remains largely neglected and underreported in various states of India. The present study was aimed to assess coxiellosisamong lactating cattle (n=134) based on their seroscreening at household level in Malappuram district of Kerala, India. On testing the cattle sera by trans-PCR targeting IS1111 gene of C. burnetii for the pathogen detection as well as by a commercial ELISA kit for the detection of antibodies against C. burnetii, the acute infection was noticed in 01 out of 134 animals (0.75%) that tested positive in trans-PCR assay while 06/134 (4.5%) animals indicated a persistent focalised infection based on their positivity in ELISA. Therefore, we conclude and recommend that the use of trans-PCR along with a serological assay like ELISA on serum samples is indispensable for early diagnosis of acute cases of coxiellosis in animals as well as to get a realistic assessment of the disease burden based on seroprevalence. This study seems to be the first epidemiological survey to highlight the hidden threat of coxiellosis among cattle population in Kerala, a southern state of India. However, other domestic and wild animals including cattle as well as their human contacts at high-risk need to be investigated through a large scale and multi-centric epidemiological study for making a realistic assessment of the associated risk factors and to unmask the zoonotic potential of C. burnetii infection in the country.
Authors and Affiliations
Pankaj Dhaka, Satyaveer Singh Malik, Jay Prakash Yadav, Manesh Kumar, Jess Vergis, Radhakrishna Sahu, Lijo John, Sukhadeo Baliram Barbuddhe, Deepak B. Rawool
Keywords
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- EP ID EP428033
- DOI 10.18006/2017.5(3).377.383
- Views 95
- Downloads 0
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