SEVA TB ELISA - Multi antigen and antibody assays for serodiagnosis of suspected cases of pulmonary and extra pulmonary tuberculosis in tertiary care hospital -A retrospective study
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2012, Vol 2, Issue 0
Abstract
Objective: To assess the usefulness of in-house developed multiantigen and antibody assays, in diagnosis of both pulmonary and extra-pulmonary tuberculosis. Method: Clinically suspected cases of 31 pulmonary and 171 extra-pulmonary tuberculosis (TB) were screened by ELISA using cocktail (ES-31 + EST-6) antigen and their specific antibodies (anti ES-31 + anti EST-6 IgG) for detection of antibody and and antigen (circulating antigen and immune complexed antigen) respectively and correlated with antituberculosis therapy in retrospective study. Results: Out of 31 cases of pulmonary TB screened, 15 patients showed ELISA positivity out of which five cases were given antituberculosis therapy. Out of 171 cases of EPTB screened, 76 cases showed ELISA positivity out of which 18 were given antituberculosis therapy. Further 4 EPTB cases which showed AFB negativity were given ATT. The data was further analyzed based on PTB & EPTB, adults and children, OPD and IPD patients to understand false positivity in clinically suspected PTB and EPTB cases. There was significant correlation (108/202 cases) with ELISA negativity and no ATT advised in clinically suspected PTB and EPTB patients. Conclusions: In house developed multi antigen and antibody assays have been observed to be quite useful as adjunct test in serodiagnosis of suspected cases of tuberculosis in particular extrapulmonary tuberculosis.
Calcium and phosphorus determination in bones of low value fishes, Sardinella longiceps (Valenciennes) and Trichiurus savala (Cuvier), from Parangipettai, Southeast Coast of India
Objective: To investigate calcium and phosphorus levels in the bones of low value fishes Sardinella longiceps (oil sardine) and Trichiurus savala (ribbon fish). Methods: Bones and skeletal remains were subjected to alkal...
Dengue and lupus: The problem of co-occurrence
Dengue is a common tropical disease. It can be seen in any tropical countries. The occurrence of dengue in the patients with underlying medical illness is very interesting and can be a complicated case. Here, the authors...
Assessment of gastroprotective effect of crude tannin from Schwenkia americana Linn. on mitochondrial functions in ulcerogenic rats
Objective: To investigate the gastroprotective effect of tannin from Schwenkia americana Linn. on mitochondrial functions in ulcerogenic rats. Methods: A total number of 36 male Wistar rats weighing 160–220 g were used f...
Evaluation of hepatoprotective activity of alcoholic and aqueous extracts of Selaginella lepidophylla
Objective: To screen Selaginella lepidophylla (S. lepidophylla) that are used in traditional medicine for their claimed hepatoprotective properties. Methods: Alcoholic and aqueous extracts of S. lepidophylla were evaluat...
An epidemiological study on the drug resistance of Escherichia coli strains isolated from women patients with urinary tract infection in Shalamzar, Iran
Objective: To investigate drug resistance of various strains of Escherichia coli (E. coli) bacteria isolated from female patients with urinary tracts infections (UTIs) in Shalamzar, Iran. Methods: This study was conducte...