Shiga Toxin and Non-Shiga Toxin-Producing Escherichia coli O157 from Cattle, Goats and Chickenin Ado-Ekiti, South West, Nigeria
Journal Title: International Journal of TROPICAL DISEASE & Health - Year 2015, Vol 6, Issue 4
Abstract
Background: Illnesses due to contaminated food, particularly food of animal origin, are perhaps the most widespread health problem in the contemporary world. Aims: To detect Shiga toxin-producing Escherichia coli (STEC) in food animals in Ado-Ekiti, Nigeria and the possible risk to human health. Study Design: Non-repeat faecal samples from various animals and poultry birds were examined for STEC. Place and Duration of Study: Department of Microbiology, Ekiti State University, Ado-Ekiti, between January 2010 and December 2011. Methodology: We investigated 722 non-repeat faecal samples from animals and poultry birds for the presence of STEC using bacteriological, serological, and tissue culture techniques. Detection of virulence genes was performed by PCR. Results: Overall, 316 isolates of E. coli were recovered from 62.3% cattle, 19.6% local chicken, 10.1% goats, 4.1% broiler, 2.9% layers, and 0.9% cockerels. Of the non-sorbitol fermenting E. coli phenotype selected from the isolates, 13.3% were presumptively identified as O157 serotype based on inability to ferment sorbitol on sorbitol MacConkey agar (SMAC). Serotyping using commercial kits capable of detecting O157 and non-O157STEC confirmed 6.6% of these as O157 comprising 4.1% from cattle and 2.5% from local chicken. Only 4.7% of the strains were serologically confirmed as non-O157 of which 0.9% was from cattle, 3.2% from goat and 0.6% from local chicken. Verocytotoxicity test and the presence of virulence genes stx1, stx2 and eae assayed by PCR showed the complete absence of virulence genes in the 13 serologically confirmed strains of O157 from cattle. The virulence gene stx1 was detected only in non-O157 strain from goat and local chickens. Conclusion: This study has shown that the prevalence of E. coli O157 is low in food animals in the study area compared to reports from the developed countries. Furthermore, our study is the first to report the isolation of non-O157STEC in goat, a very common domestic animal, in the study area.
Authors and Affiliations
A. O. Oluyege, O. Famurewa
Keywords
Related Articles
- EP ID EP343358
- DOI 10.9734/IJTDH/2015/7352
- Views 80
- Downloads 0
Currently Observed Trend in the Resistance of Malaria to Artemisinin Based Combination Therapy in Nigeria – A Report of 5 Cases
Malaria is a life threatening disease caused by parasites transmitted through the bite of an infected female mosquito. About 3.2 million (about half of the world’s population) are at risk of malaria; it affects all age g...
An Analysis of Seasonal Variation and Climatic Influences on the Male to Female Ratio of Live Births in Malta
Aims: In humans, male births occur in excess of female births. The ratio of male births to total births (MFR) is expected to approximate 0.515. Many factors have been shown to influence MRF and a seasonal pattern has als...
Association of Self-perception Lifestyle with Overweight / Obesity among People with a Family History of Type 2 Diabetes: A Cross Sectional Study
Aim: To analyze the association between self-perception of lifestyle and overweight / obesity among people with diabetic parents. Study Design: Cross-sectional, analytical study. Place and Duration of Study: It is a comm...
Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu lato
Aims: The aim of this review is to present Rickettsioses sensu lato, with emphasis on their current and future clinical diagnosis. The review presents the conditions, the agents that cause them, and the current gold stan...
Anti-plasmodial Activity of Bee Sting in Plasmodium berghei Infected Mice
Aim: Based on traditional claims and practice, the antiplasmodial activity of bee stings and its effect on haematological indices was investigated in P. berghei infected mice. Methodology: Sixteen albino mice were intra...