Shoot multiplication and direct organogenesis of an important medicinal plant Plumbago zeylanica L. (Plumbaginaceae)

Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 6


Efficient micropropagation protocol was developed for Plumbago zeylanica L., a species threatened due to over exploitation for medicinal purposes and habitat destruction in Southern Peninsular India. Multiple shoot induction was more successful using nodes as explants on Murashige and Skoog’s (MS) medium supplemented with 1mg/L benzyl amino purine (BAP) . Shoots, when transferred to MS medium containing 0.2 – 0.5 mg/L gibberellic acid (GA3) showed variable elongation. Further, MS medium fortified with 1.5 mg/L BAP induced highest frequency of shoots through adventitious de novo organogenesis. Shoots developed were rooted on full strength MS medium with either α-naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA). Optimum shoots and root multiplication were obtained within 8 weeks. In vitro derived plantlets were successfully weaned and transferred to soil and showed 90 % survival rate

Authors and Affiliations

Lubaina AS, Nair GM, Murugan K


Related Articles

Epidemiological studies on hospital based incidence of Japanese encephalitis in the Jharkhand state of India

Study was conducted in Rajendra Institute of Medical Science (RIMS), Ranchi, Jharkhand, during June 2012 to September 2013. The objective of the study was to know the hospital based incidence of Japanese Encephalitis (JE...

In vitro and in vivo potentiation of ampotericin-B by flavonoid against different fungal strains

Synergistic effects of 18 flavonoids (11 glycosides and flavones, 01 flavones diglycoside, 04 chalcones and 02 aglycones) in combination with different anti-fungal agents against fungal strains were investigated. The aga...

"Gregariousness" behavior of little grebe Tachybaptus ruficollis (Pallas, 1764) at Morocco.

This study aims to identify the "gregariousness" behavior of the little grebe Tachybaptus ruficollis at Morocco from 2013 to 2014 in the hilly reservoir of Hassar dam in the province of Mohammedia at Morocco. The number...

Evaluation of air pollution tolerant tree species for Kothagiri Municipal Town, the Nilgiris, Tamil Nadu

Air pollution tolerance index (APTI) has been determined by pooling the at-tributes viz., total chlorophyll, ascorbic acid and moisture content of leaves and leaf extract pH for certain locally available tree species in...

Laboratory toxicity evaluation of Diflubenzuron, a chitin-synthesis inhibitor, against Anopheles darlingi (Diptera, Culicidae)

We evaluated Diflubenzuron toxicity against larvae and pupae of Anopheles darlingi. A series of bioassays were developed to assess the lethal concentrations LC50 and LC90 to larvae after two exposure periods (24 and 48 h...

Download PDF file
  • EP ID EP563236
  • DOI -
  • Views 28
  • Downloads 0

How To Cite

Lubaina AS, Nair GM, Murugan K (2011). Shoot multiplication and direct organogenesis of an important medicinal plant Plumbago zeylanica L. (Plumbaginaceae). Journal of Research in Biology, 1(6), 424-428.