Significance of Glycosylation Changes in Oral Cancer
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2015, Vol 17, Issue 2
Abstract
Oral cancer is the major health burden in India. Glycosylation changes are associated with malignant transformation. Therefore, we investigated alterations in several glycoconjugates in oral cancer. Total 50 healthy controls and 50 oral cancer patients were enrolled for the study. Serum total sialic acid (TSA) and fucose were analyzed using spectrophotometric method, serum glycoprotein profile was performed by native PAGE followed by glycoprotein staining and for ST3GALI and FUT6 mRNA expression, reverse transcriptase PCR (RT-PCR) was performed. The result depicted that TSA/TP ratio and fucose were found to be increased in oral cancer patients as compared to the controls. Receiver Operating Curve (ROC) analysis indicated that fucose/TP ratio could significantly distinguish between controls and oral cancer patients. Fucose/TP ratio was found to be higher in tobacco habituates oral cancer patients as compared to non-habituates controls and habituates controls. ST3GAL1 mRNA expression was found to be higher in malignant tissues as compared to normal tissues. The mRNA expression of FUT6 was comparable between malignant and adjacent normal tissues. The glycoprotein profile showed an increase in 44 kDa in oral cancer patients as compared to the controls. Moreover, levels of 230 kDa glycoprotein were observed to significantly increased in oral cancer patients as compared to controls. The study illustrated that glycosylation has significant role in development of oral cancer. Further in-depth studies with a larger sample size can give insights into the molecular mechanism underlying glycosylation in oral cancer
Authors and Affiliations
Jashvanti Radhvani, Bhairavi Vajaria, Prabhudas Patel
Keywords
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