Significance of Variable Number Tandem Repeats (VNTR) Polymorphism of Thymidylate Synthase Gene in Patients with Colorectal Cancer: A Preliminary Study
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2015, Vol 17, Issue 2
Abstract
5-fluorouracil (5-FU) is the most frequently used chemotherapeutic agent in the treatment of colorectal cancer (CRC) both in adjuvant and in palliative therapy. This drug mainly acts by inhibiting the activity of its target enzyme thymidylate synthase (TS) which leads to DNA damage. TS gene contains variable number of 28 bp tandem repeats (VNTR) in the 5’UTR region. This VNTR polymorphism can alter the TS levels. As the response of 5-FU is dependable on the level of TS in the cells, TS VNTR polymorphism could be an informative tool in clinical handling of CRC patients being treated with 5-FU based therapy. Therefore, the aim of the present study was to determine the significance of TS gene VNTR polymorphism in patients with colorectal cancer. In this retrospective study, 40 untreated CRC patients were included. TS gene VNTR polymorphism was studied using PCR technique. The data was analyzed statistically using SPSS software. There was predominance of 2R/3R heterozygous genotype by 53% (21/40) as compared to 2R/2R and 3R/3R homozygous genotype, 22% (9/40) and 25% (10/40), respectively. Patients with 2R/2R genotype had significantly higher rate of recurrence as compared to other two genotypes. However, a larger cohort and longer follow-up will be required for final conclusion.
Authors and Affiliations
Nirzary Bhatt, Kinjal Gajjar, Trivedi Trupti, Darshita Patel, Nandita Ghosh
Rising Tobacco Related Cancers !!! – Issues and our Role
World: Every year around 14 million new cancer cases are diagnosed across the globe.1 Around 8.2 million cancer related deaths occurred in 2012. And it is expected that annual cancer cases will rise from 14 million in 20...
Meig’s Syndrome - A Diagnostic Dilemma
Meig’s syndrome is a benign condition with 100% cure rates. However this condition mimics advanced ovarian malignancy, causing diagnostic dilemma to the treating doctor. It is a rare condition and seen only in 1% cases o...
FLT3 Protein Expression and Mutation in B Cell Acute Lymphoblastic Leukemia (B-ALL)
FLT3 is a receptor tyrosine kinase, which play important role in normal hematopoiesis activating mutations of FLT3 are commonly found in acute leukemia patients and reported to be associated with poor clinical outcome. T...
Dialogue, Discussion and Debate
Whenever learned people assemble, there is dialogue, discussion or debate as they think and deliberate on important issues. And every dialogue, discussion or debate adds to our understanding of fundamentals. Do you know...
Summaries
Summaries of Presentations at Clinical Meetings