Significance of Variable Number Tandem Repeats (VNTR) Polymorphism of Thymidylate Synthase Gene in Patients with Colorectal Cancer: A Preliminary Study
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2015, Vol 17, Issue 2
Abstract
5-fluorouracil (5-FU) is the most frequently used chemotherapeutic agent in the treatment of colorectal cancer (CRC) both in adjuvant and in palliative therapy. This drug mainly acts by inhibiting the activity of its target enzyme thymidylate synthase (TS) which leads to DNA damage. TS gene contains variable number of 28 bp tandem repeats (VNTR) in the 5’UTR region. This VNTR polymorphism can alter the TS levels. As the response of 5-FU is dependable on the level of TS in the cells, TS VNTR polymorphism could be an informative tool in clinical handling of CRC patients being treated with 5-FU based therapy. Therefore, the aim of the present study was to determine the significance of TS gene VNTR polymorphism in patients with colorectal cancer. In this retrospective study, 40 untreated CRC patients were included. TS gene VNTR polymorphism was studied using PCR technique. The data was analyzed statistically using SPSS software. There was predominance of 2R/3R heterozygous genotype by 53% (21/40) as compared to 2R/2R and 3R/3R homozygous genotype, 22% (9/40) and 25% (10/40), respectively. Patients with 2R/2R genotype had significantly higher rate of recurrence as compared to other two genotypes. However, a larger cohort and longer follow-up will be required for final conclusion.
Authors and Affiliations
Nirzary Bhatt, Kinjal Gajjar, Trivedi Trupti, Darshita Patel, Nandita Ghosh
Keywords
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