Sildenafil May Have an Inhibition Effect on The TGFβ1-Smad2/3 Pathway in PAH Rats
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 15, Issue 2
Abstract
Pulmonary artery remodeling plays a key role in the development of Pulmonary Artery Hypertension (PAH). And the TGF-β1-Smad2/3 pathway takes part in this pathological process. It is well known that sildenafil improved the PAH by inhibiting the PDE5.However whether sildenafil could inhibit the TGF-β1-Smad2/3 pathway is unknown. Thus, sixty rats were randomly divided into a control group (Control), a model group (MCT) and a sildenafil group (MCT+SILD). MCT solution (60mg/kg) was subcutaneously injected to establish the model of Pulmonary Artery Hypertension in the MCT and MCT+SILD groups. Treatment with sildenafil (50mg/kg) was orally administrated from the 22nd day for 2 weeks. At the end of the 5th week, the Right Ventricular Systolic Pressure (RVSP) was obtained, and the Right Ventricular Hypertrophy Index (RI) was determined as the ratio of the right ventricular weight to the left ventricular plus septal weight (RV/LV+S). Western blots were used to determine the expression of TGFβ1, Smad2/3, P-Smad 2/3 and matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9(MMP9) in lung tissues. Results showed that TGFβ1, Smad2/3, P-Smad 2/3, MMP2 and MMP9 expressions, as well as RVSP and RI, were decreased in the sildenafil treatment group. Sildenafil may have an inhibition effect on the TGFβ1-Smad2/3 pathway in rats.
Authors and Affiliations
Yijia Xiang, Yonghui Wu, Shiyong Ye, Chunlai Zeng
Keywords
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- EP ID EP587809
- DOI 10.26717/BJSTR.2019.15.002669
- Views 152
- Downloads 0
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