Simple and rapid identification of the mycobacterium tuberculosis complex by immunochromatographic assay using MPT 64 antigen detection test
Journal Title: MedPulse -International Medical Journal - Year 2016, Vol 3, Issue 9
Abstract
Background: In developing countries like India, more than 90% of tuberculosis infections are still caused by M. tuberculosis. Phenotypic and genotypic methods used for identification of mycobacterial species are either cumbersome or expensive. The Mycobacterium tuberculosis protein 64 (MPT-64) antigen is an M. tuberculosis complex (MTBC) specific antigen and immunochromatographic test based on this Ag has been used to identify MTBC rapidly. The present study was undertaken to evaluate this commercial assay for characterization of Mycobacteria. Material and Methods: A total of100 isolates recovered from both pulmonary (50 isolates) as well as extra pulmonary specimens (50 isolates) were characterized as MTBC or NTM based on standard phenotypic characteristics and biochemical tests. These results were compared with commercial SD TB Ag MPT64 Rapid test. Results: Among 100 mycobacterial isolates, 98 isolates were identified as MTBC and the two isolates from extra pulmonary cases were identified as NTM on the basis of biochemical tests. There was no discrepancy found in differentiation of these isolates between biochemical tests and TB Antigen MPT64 rapid test. Conclusion: The sensitivity and specificity of TB Antigen MPT64 rapid test was 100% as compared to biochemical methods. This commercial assay is rapid, simple, easy to perform, interpret and economical with high sensitivity and specificity to identify MTBC from culture isolates.
Authors and Affiliations
Prashant Renake, Shripad Taklikar
Keywords
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