SIMPLE METHOD OF ISOLATION OF LOGANIN FROM NUX VOMICA FRUITS AND ITS QUANTIFICATION BY HPLC
Journal Title: International Journal of Pharmacognosy - Year 2017, Vol 4, Issue 1
Abstract
Loganin is an iridoid glycoside, a key phytoconstituent in Strychnos plants having a crucial role in biosynthesis of indole, monoterpene alkaloids and other natural products. It is a bitter iridoid glycoside with numerous activities like anti-inflammatory, anti-oxidant, glucose-lowering and anti-diabetic nephropathy activities. The objective of this study was to develop a simple method for isolation of loganin from fruit pulp of Nux vomica and quantify by HPLC. The isolated compound was characterized by TLC, UV, IR, MS and NMR spectral analysis and purity was confirmed by HPLC analysis. Quantification of loganin was done from fruit, seeds and pulp extract using HPLC.A simple solvent partitioning method was used for extraction and isolation process. This isolation method developed involved easy solvent-solvent extraction technique followed by recrystallization to purify the compound.
Authors and Affiliations
S. P. Patil
Keywords
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