Simple Spectrophotometric Method for Analysis of Serum Catalase Activity
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 9
Abstract
ABSTRACT Introduction: Catalase is a principal constituent of the antioxidant system that attenuates the oxidative stress, which is ubiquitously associated with several types of pathological disorders. Aim: This paper describes a discontinuous assay for the assessment of catalase activity using the titanium tetrachloride/sulfuric acid reagent. Materials and Methods: Samples containing catalase are incubated with hydrogen peroxide for three minutes prior to fast mixing of aliquots of the incubation mixtures with titanium tetrachloride/sulfuric acid reagent, which measures remaining hydrogen peroxide (H2O2). Absorbance is then read at 405 nm. Dissociation of hydrogen peroxide is proportional to catalase activity in the used sample. One-way analysis of variance (ANOVA) was used to analyse the resulted data. p-value <0.05 was considered to be statistically significant using SPSS Statistics 17.0 software. Results: The current method characterizes the use of a correction factor to exclude the interference that arises from the presence of a yellow background colour of serum and/or reducing agents that reduce titanium (IV) to titanium (III). The imprecision of the method was considered by calculating the coefficient of variation, which equals to 3.6% within run and 5.7% between run. The catalase assay measured using the kinetic method produced a good correlation (r=0.9771). Conclusion: The study explains a simple discontinuous method for catalase activity assessment which can be completed with few steps, and which allows catalase to be measured in the presence of high concentration of other molecules as well as at low levels of H2O2.
Authors and Affiliations
Mahmoud Hussein Hadwan, Haider Khabt
Keywords
Related Articles
- EP ID EP522845
- DOI 10.7860/JCDR/2018/35014.12011
- Views 526
- Downloads 0
Comparative Analysis of Colour Doppler Ultrasonography and CT Angiography in Peripheral Arterial Disease: Studying effects of Vessels Wall Calcification on Diagnostic Evaluation
ABSTRACT Introduction: Peripheral Arterial Disease (PAD) is one of the most common causes of morbidity in diabetes mellitus in Indian population. Aim: To analyse comparative role of CDUS (Colour Doppler Ultrasonography)...
Successful Management of Traumatic Rupture of the Inferior Rectus Muscle
A 25-year-old male patient presented with diplopia after a penetrating injury to the right eye. Examination revealed right hypertopia and limitation of infraduction of right eye with rupture of Inferior Rectus (IR) muscl...
Evaluation of HiaureusTM Coagulase Confirmation Kit in Identification of Staphylococcus aureus
Introduction: Staphylococcus aureus is a facultative anaerobic Gram positive coccal bacterium whose incidence ranges to different infections. It is a cause of various uncomplicated skin infections, abscesses, septicaemia...
Intramuscular Capillary Haemangioma of the Temporalis Muscle: A Rare Case with A Review of the Literature
An Intramuscular Haemangioma (IMH) is a benign mesenchymal tumour of the endothelial cells that accounts for less than 1% of all haemangiomas. Here we report the case of a capillary type intramuscular haemangioma in a fi...
Antimicrobial Efficacy of Garlic-Lemon in Comparison with Sodium Hypochlorite against E. faecalis
ABSTRACT Introduction: Sodium hypochlorite is the most commonly used root canal irrigant in endodontics. Although, it is a highly effective antimicrobial agent, it is highly cytotoxic. This study has focused on search of...