Simplification of Molecular Methods
Journal Title: Journal of Diagnostic Techniques and Biomedical Analysis - Year 2022, Vol 11, Issue 1
Abstract
We have simplified and improved few techniques in furthering applications of molecular methods and products. This has been largely achieved by making them simple, ambient stable, rapid and cost effective. In this comprehensive article, beside simplification of few methods, we are reporting on devices like a “home-made cheap ultra-rapid thermal cycler” and a “homemade cheap and efficient Portable Transformation Device” for high efficiency transformation of one millilitre of fresh as well as stored cultures. Included in this paper are methods of extended storage of DNA at Room temperature in a ready-to-use form and for repeated utilization; development of ambient stable, rapid and cheapest in the world DNA Diagnostic kit for identification of micro-organisms; plant sex determination at nursery stage under Field as well as laboratory conditions. The simple kit can be used even by semiskilled personnel. Also reported here are sensitive and rapid detections from clinical samples; cheap and easy methods of DNA synthesis; easy, cheap, rapid and large scale DNA isolations. These methods can have wide applications in detection of infection and surveillance during outbreaks, pandemics, and as a routine exercise in the Border areas impending threat of Biological Warfare.
Authors and Affiliations
M. Giredhar, Ram Singh, B. S. Karotia, A. Chakraborty and S. S. Lahiri
Keywords
Related Articles
- EP ID EP741651
- DOI -
- Views 28
- Downloads 0
In-house Modification and Improvement of the CDC Realtime PCR Diagnostic Assay for SARS-CoV-2 Detection
The world is currently facing an unprecedented pandemic caused by the novel coronavirus SARS-CoV-2 (COVID-19) which was first reported in late 2019 by China to the World Health Organization (WHO). The containment str...
Infectious Realities: Navigating the World of Contagions
Infectious diseases, a persistent facet of the human experience, weave a narrative of contagion that spans centuries, continents, and civilizations. "Infectious Realities: Navigating the World of Contagions" invites u...
Molecular Diagnostics: Decoding Disease at the Genetic Level
"Molecular Diagnostics: Decoding Disease at the Genetic Level" represents a cutting-edge and transformative approach in the field of medical diagnostics. This innovative discipline utilizes the power of molecular biology...
From Data Streams to Diagnoses: AI's Journey in Healthcare
"The Role of Artificial Intelligence in Disease Diagnosis" marks a paradigm shift in the landscape of healthcare, imposing advanced technologies to enhance accuracy, efficiency, and personalized patient care. Artifici...
Simplification of Molecular Methods
We have simplified and improved few techniques in furthering applications of molecular methods and products. This has been largely achieved by making them simple, ambient stable, rapid and cost effective. In this com...