Simultaneous Detection of Methicillin- and Mupirocin-Resistant genes in Staphylococcus aureus Isolated with Multiplex PCR from Patients in the City of Darab, Fars Province, Iran
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 12
Abstract
Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens in hospitals. Hospital staffs can be one of the main sources of this organism in nosocomial infections. Identification and control of colonized health care workers can reduce the incidence of methicillin-resistant Staphylococcus aureus (MRSA). Mupirocin is a topical antibiotic and inhibits the activity of the most of grampositive Cocci. Mupirocin resistance emerges in a short time after taking this antibiotic. The main goal of this study is to evaluate the prevalence of asymptomatic carriers of methicillin- and mupirocin-resistant Staphylococcus aureus by Multiplex PCR method among Fasa Hospital Staffs, Fars Province, Iran. Materials and Methods: In this study, 150 samples of Staphylococcus aureus isolated from the hospital staffs were analyzed and confirmed by biochemical and microbial methods. Disk diffusion method was used to determine the level of strains susceptibilities toward methicillin and mupirocin. Then the presence of mec-A, mup, nuc genes was examined using Multiplex PCR. 150 people were included in this study, including 120 women and 30 men. Results: Of the 150 studied people, 53 cases including 42 women and 11 men were Staphylococcus aureus carriers. Four individuals among 11 studied men were resistant to methicillin and none of them was resistant to mupirocin. A number of 11 cases among 42 studied women were resistant to methicillin and 2 women were resistant to mupirocin. Conclusion: Prevalence of methicillin- and mupirocin-resistant Staphylococcus aureus among Fasa Hospital Staffs was moderate and relatively low. Permanent control of carriers and their treatment can prevent spread of the bacteria and those infections caused by them.
Authors and Affiliations
Ghasem Nikfar| Department of Pediatrics, School of Medicine, Fasa University of Medical Sciences, Fasa,Iran, Houshang Jamali| Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran CorrespondingEmail:h.jamali1970@gmail.com, Jasem Ahmadzadagan| Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Keywords
Related Articles
- EP ID EP12234
- DOI -
- Views 275
- Downloads 12
The Mechanical Behavior Variation of Nickel - Titanium Orthodontic Wires in Different Fluoride Mouthwash
In this study, an orthodontic arch wire of Ni-Ti alloy made by Germany in four different mouthwashes contain fluoride was investigated in mechanical behavior. This research focused on the mechanical behavior changes in...
Assessment the relationship between Testis Cancer incidence and mortality rate with human development index in the European countries in 2012
Testis Cancer (TC) is the most common cancer in 15-39 year-old men and with the white Caucasian race. This cancer consists of 0.7 percent of men’s cancer all over the world. The aim of this study is to investigate the...
Efficacy of harmonic scalpel in total clipless laproscopic cholecystectomy
Laparoscopic cholecystectomy (LC) is a well-known benchmark in the surgical management of gall bladder stones. It has numerous advantages such as its minimal invasiveness and faster recovery for patients. Harmonic scalp...
Prevalence of AmpC type extended spectrum beta lactamases genes in clinical Samples of E.coli Isolated from Poultry and Humans
Emergence of antibiotic resistance among pathogens, particularly in health centers and hospitals, has become a major public health problem. This study identified AmpC-type beta-lactamase against the antibiotic ceftazidi...
Clinical, Microbiological and Radiologic evaluation of topical use of Co-Amoxiclav 25% dental gel in the treatment of adult periodontitis
The aim of this study was to compare the effect of the topical application of co-amoxiclav gel with placebo in the treatment of adult periodontitis by conducting clinical, microbiological and radiologic measurements. A...