Simultaneous detection of respiratory viruses using multiplex PCR method in patients with upper and lower respiratory tract infection
Journal Title: Ege Tıp Dergisi - Year 2015, Vol 54, Issue 1
Abstract
Aim: In this study, we aimed to determine the respiratory viruses in a short time by multiplex PCR method in patients with upper and lower respiratory tract infections. Materials and Methods: In this report, between January 2007 and August 2013, a total of 1708 patients (1220 children, 488 adult; 45,1% female, 54.9% male, age range: 5 days-94 years) prediagnosed as having upper or lower respiratory tract infections were enrolled in the study. Respiratory viruses were investigated by multiplex PCR method in respiratory samples of these patients. Results: In a total of 1708 patients, 620 (36.3%) of them were positive for one or more respiratory viruses and 1088 (63.7%) were negative. In 63 of the patients (3.7%) multiple viruses were detected. Most of the samples (89.5%) containing multiple agents were detected in children. Respiratory specimens were obtained from 387 (22.7%) outpatients and 1321 (77.3%) inpatients. One or more respiratory viruses were detected in 39.2% of pediatric patients, 29.1% of adult patients (p<0.000), 48.5% of outpatients and 32.7% of inpatients. In all samples, influenza A(H1N1)pdm09 virus, RSV, rhinovirus and influenza A virus were found be positive in 12.1%, 9.0%, 3.7% and 3.0%, respectively. Conclusion: Respiratory viruses were positive 40% in patients with acute respiratory tract infections. Multiple viral infections were detected in approximately 4% patients. Almost 90% of these patients were found to be of the pediatric patients. If influenza A(H1N1)pdm09 virus is kept separate, the three most common pathogen were RSV, rhinovirus and influenza A virus, respectively. Respiratory viruses were detected more frequently in children compared to adult patients and in outpatient compared to hospitalized patients.
Authors and Affiliations
Candan ÇİÇEK, Ayşe ARSLAN, Eylem Ulaş SAZ, Hüsnü PULLUKÇU, Gürsel ÇOK
Keywords
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- EP ID EP343005
- DOI 10.19161/etd.344100
- Views 90
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