Simultaneous determination of artificial sweeteners in possible counterfeited wines, using high performance liquid chromatography with DAD detection
Journal Title: Ovidius University Annals of Chemistry - Analele Universităţii "Ovidius" Constanţa. Seria chimie - Year 2012, Vol 23, Issue 1
Abstract
Increased use of food, beverages or drugs containing synthetic sweeteners presents a real danger to health, which is why the EU Member States had to establish a system of regular surveys to monitor sweetener consumption. In case of wine industry, according to law no. 244/2002, Romania prohibits the addition of synthetic sweeteners in wine in order to obtain sweet wine. The official method for detection of adulterated sweet wines with synthetic sweeteners is TLC-Thin Layer Chromatography. However, quantitative methods of analysis are needed to measure levels of sweeteners in different food matrices and high performance liquid chromatography has proved to be a powerful tool for quantitative analysis of compounds at traces levels. In this paper, a high performance liquid chromatographic (HPLC) method for the simultaneous separation and determination of three of the most popular artificial sweeteners (acesulfame potassium, saccharine and aspartame) in a single injection was developed. The described method is rapid, accurate and highly sensitive. Detection limit were 4 mg/L for acesulfame K, 1 mg/L for saccharine and 9 mg/L for aspartame respectively. The precision of the method was about 2% and recovery ranged between 92.6% and 103.3%. There were analyzed commercial wine samples, in order to detect possible counterfeits-sweet and medium sweet wines. Therefore, of the 20 analyzed wine samples, only two samples consisting in wine sweet table, were counterfeited by adding saccharin.
Authors and Affiliations
GEANA Elisabeta-Irina, IORDACHE Andreea-Maria, IONETE Roxana Elena
Keywords
Related Articles
- EP ID EP98673
- DOI -
- Views 141
- Downloads 0
Nontemplate synthesis, characterization and theoretical study of tetraazamacrocycles
The syntheses of new tetraaza macrocyclic compounds of variable ring sizes by non-template methods and their characterization with the help of elemental analysis and spectroscopic techniques (FT-IR, [sup]1[/sup]H-NMR, an...
Study of density and viscosity for ternary mixtures biodiesel+diesel fuel + bioalcohols
The increase of the environment pollution, together with the instable price of crude oil led in the last years to a renewed focus on biofuels. As the demand in the transport sector is continuously increasing, and taking...
Simultaneous determination of artificial sweeteners in possible counterfeited wines, using high performance liquid chromatography with DAD detection
Increased use of food, beverages or drugs containing synthetic sweeteners presents a real danger to health, which is why the EU Member States had to establish a system of regular surveys to monitor sweetener consumption....
Environmental pollution in the petroleum refining industry
The petroleum refining industry has a significant influence on the total pollution of the environment by industrial discharges and wastes. In the operation of petroleum refineries, the atmosphere is polluted with hydroge...
A study of rheological behavior for refined rapeseed oil
The refined rapeseed oil were carefully studied in recent years because they may constitute a raw material for biodegradable lubricants getting organic. These oils are an alternative to synthetic mineral oils. This artic...