Simultaneous Determination of Caffeine, Catechin, Epicatechin, Chlorogenic and Caffeic Acid in Cola nitida Dried Nuts from Côte d’Ivoire Using HPLC
Journal Title: Asian Journal of Biotechnology and Bioresource Technology - Year 2017, Vol 1, Issue 1
Abstract
Aims: A simple high performance liquid chromatographic analysis (HPLC) for Cola nitida caffeine, catechin, epicatechin, chlorogenic and caffeic acid with a gradient system elution system was developed. Study Design: Mature kola seeds were collected in October 2014-February 2015 in South of Côte d’Ivoire. Harvested kola nuts were transferred to the laboratory until used in the experiments. Place and Duration of Study: This study was carried out during the year 2016 at European institute of membranes, France. Methodology: Kola nuts was extracted from mature kola seeds (Cola nitida Schott & Endl.) and the extract was obtained by infusion of kola nut powder in water-ethanol mixture at room temperature. The compounds were separated by of a C18 reversed-phase column with a gradient elution system of binary phase consisted of A (95/5, water-H2O/methanol-MeOH + 0.1% trifluoroacetic acid-TFA) and B (100% acetonitrile-ACN + 0.1% trifluoroacetic acid-TFA) and an UV detector. All of these compounds were separated within 70 min. The validity of this method was confirmed by their quantitative measurement in kola samples. Results: The limit of detection (LOD) and the limit of quantification (LOQ) of these compounds were within the range of 0.098 to 0.47 µg/mL and 0.3 to 1.45 µg/mL, respectively. All the analyses exhibited good linearity with correlations coefficients above 0.9974 and the accuracies for the analyses were 97 – 104%. Conclusion: Using this analytical method, the bioactive compounds of kola nuts have been determined with satisfactory. The presence of these compounds (caffeine, catechin, epicatechin, cholrogenic acid and caffeic acid) in the extract justifies the industrial interest of kola nuts.
Authors and Affiliations
Yves Nyamien, Adama Coulibaly, Marie-Pierre Belleville, Eddy Petit, Augustin Adima, Godi Henri Biego
Keywords
Related Articles
- EP ID EP337879
- DOI 10.9734/AJB2T/2017/34800
- Views 94
- Downloads 0
Phytochemical Components, Antioxidant and Cytotoxic Activities of Mulberry Mistletoe (Loranthus parasiticus Merr) Leaves Extracts
Herein, we investigated the effects of solvents on the phytochemical components, antioxidant activities, and cytotoxicity of Mulberry mistletoe leaves. An FT-IR method was performed to identify the essential functional g...
Effect of Processing Methods on the Quality of Biscuit Supplemented with Pigeon Pea Seed Flour
Aim: To determine the effect of toasting, boiling, germination and fermentation of pigeon pea seeds on the quality of biscuit supplemented with pigeon pea flour. Design of Study: The experiment was carried out in 3 repli...
Simultaneous Determination of Caffeine, Catechin, Epicatechin, Chlorogenic and Caffeic Acid in Cola nitida Dried Nuts from Côte d’Ivoire Using HPLC
Aims: A simple high performance liquid chromatographic analysis (HPLC) for Cola nitida caffeine, catechin, epicatechin, chlorogenic and caffeic acid with a gradient system elution system was developed. Study Design: Mat...
Plasmid – mediated Resistance in Salmonella typhi Isolates from Door Handles in Nasarawa State, North-central Nigeria
Contamination of door handles with antibiotic resistant bacteria can be a major threat to public health, as the antibiotic resistant determinants can be transferred to other pathogenic bacteria thus, compromising the tre...
Trends in Treatment and Vaccine Development of Typhoid Fever: A Review
Typhoid fever is caused by bacterium called Salmonella enterica serovar typhi (often referred to as S. typhi). It has become rare developed countries, but remains an important cause of enteric disease in developing count...