Simultaneous Determination of Hydrochlorothiazide and Irbesartan from Pharmaceutical Dosage Forms with RP-HPLC
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2020, Vol 17, Issue 5
Abstract
Objectives: In this work, a simple and rapid liquid chromatographic method for the simultaneous determination of irbesartan (IRBE) and hydrochlorothiazide (HCT) was developed and validated by reverse phase high performance liquid chromatography (RP-HPLC). Materials and Methods: Experimental conditions such as different buffer solutions, various pH values, temperature, composition of the mobile phase, and the effect of flow rate were optimized. Results: The developed RP-HPLC method for these antihypertensive agents was wholly validated and IRBE was detected in the linear range of 0.1-25 μg mL-1 and HCT was detected in the linear range of 0.25-25 μg mL-1. Moreover, the suggested chromatographic technique was successfully applied for the determination of the drugs in human serum and pharmaceutical dosage forms with limit of detection values of 0.008 μg mL-1 for IRBE and 0.012 μg mL-1 for HCT. Conclusion: The proposed rapid analysis method of these antihypertensive drugs can be easily used and applied by pharmaceutical companies for which the analysis time is important.
Authors and Affiliations
Sevinç KURBANOĞLU, Aysu YARMAN
Keywords
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- EP ID EP689995
- DOI 10.4274/tjps.galenos.2019.76094
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