SIMULTANEOUS DETERMINATION OF MOXIFLOXACIN HYDROCHLORIDE AND DIFLUPREDNATE BY RATIO DERIVATIVE SPECTROPHOTOMETRY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: Ratio derivative spectrophotometric method has been developed for the simultaneous determination of Moxifloxacin HCl (MH) and Difluprednate (DIFLU) in Phosphate Buffer pH 7.4.Methods: In this method, the overlapping spectra of MH and DIFLU were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of MH and DIFLU were measured at λmax 325.8 and λmax 238.0 nm, respectively for their quantification. MH and DIFLU were determined in the concentration range of 2-10 μg/ml and 4-20 μg/ml respectively.Results: The method was validated as per the ICH guideline and accuracy, precision are found to be within the acceptable limit. The limits of detection and quantitation were found to be 0.1144 and 0.3466 μg/ml, respectively for MH and 0.0311 and 0.094 μg/ml, respectively for DIFLU.Conclusion: The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and does not require separation of MH and DIFLU hence successfully applied for simultaneous estimation of MH and DIFLU in marketed eye drops (liquid dosage form).Â
Authors and Affiliations
Ankit Soni, Mukesh Gohel, Vaishali Thakkar, Lalji Baldaniya, Tejal Gandhi
Keywords
Related Articles
- EP ID EP579735
- DOI -
- Views 53
- Downloads 0
Determination of tDETERMINATION OF 3D STRUCTURE OF GAG POLY PROTEIN ISOLATE 90CF056 OF HIV TYPE 1 BY HIDDEN MARKOV MODEL AND NEURAL NETWhree dimensional structure of Gag Poly Protein isolate 90CF056 of HIV type 1 by Hidden Markov Model and neural networks
Introduction: The study of understanding the structural and molecular conservation of HIV-1 Gag function has revealed a number of potential Gag-related targets for possible therapeutic intervention. In this study, we emp...
RAPID SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACT OF ACHYRANTHES ASPERA AND STUDY OF THEIR ANTIMICROBIAL AND FREE RADICAL SCAVENGING ACTIVITIES
Objective: To study the biosynthesis of silver nanoparticles (AgNPs) using the aqueous leaf extract of Achyranthes aspera and check the antimicrobial and free radical scavenging activity of the biosynthesized AgNPsMethod...
ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS
Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential...
THE ROLE OF HONEY WITH ROYAL JELLY IN PROTECTING THE GRAAFIAN FOLLICLES FROM THE TOXICITY OF THE ADRIAMYCIN DRUG
Objective: This study aims to investigate the ability of certain mixture of honey with royal jelly (H+R) to prevent the toxic side effects, caused by the Adriamycin drug. One of the most important side effects of this dr...
SELECTIVE MERCURIMETRIC TITRATION ASSAY OF CHLORIDE CONCENTRATION IN THE WATER OF GREEN COCONUTS USING NOVEL INDICATOR SYSTEM
Objective: A selective mercurimetric titration procedure is proposed for the assay of chloride concentration in the water of green coconut using mercury(II) nitrate [(Hg(NO3)2] reagent and iron(III) nitrate [Fe(NO3)3] wi...