SIMULTANEOUS DETERMINATION OF TARTRAZINE AND BRILLIANT BLUE IN FOODSTUFFS BY SPECTROPHOTOMETRIC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: Objective of the study was to develop a simple spectrophotometric method for simultaneous determination of two colorants, Tartrazine (T) and Brilliant Blue (BB), in foodstuffs without any prior treatment.Methods: Derivative spectrophotometric (DS) and spectrophotometric methods were applied for the determination of (T) and (BB), respectively.Results: Tartrazine was determined by first derivative spectrophotometry at 454 nm (1D454); where Brilliant blue was determined by zero-order spectrum at 630 nm. Linearity ranges were 2-24 ðœ‡g/ml for (T) and 2-14 ðœ‡g/ml for (BB), regression analysis showed a good correlation co-efficient R2=0.9999 and R2=0.9998 for (T) and (BB), respectively. The limit of detection (LOD) and limit of quantification (LOQ) was to be 0.12 and 0.35 ðœ‡g/ml for (T), 0.17 and 0.52 ðœ‡g/ml for (BB), respectively.Conclusion: The proposed methods were successfully applied to analysis individual or mixture of Tartrazine and Brilliant Blue in foodstuffs. All studied samples showed dye levels conformity with Syrian legislation. Â
Authors and Affiliations
Saad Antakli, Leon Nejem, Sheren Katran
Keywords
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