Simultaneous Estimation of Atazanavir & Ritonavir in API & Marketed Formulations by Using RP-HPLC
Journal Title: International Journal of Current Science Research and Review - Year 2024, Vol 7, Issue 10
Abstract
Development and validation of RP-HPLC method for simultaneous estimation of Atazanavir and Ritonavir in their combined tablet dosage form. Atazanavir and Ritonavir are antiviral agents used in treatment of HIV. A simple, precise, rapid, accurate and cost-effective high- performance liquid chromatography (HPLC) method was successfully developed and validated for simultaneous estimation of Atazanavir and Ritonavir in their combined tablet dosage form. The selected mobile phase was Methanol: Phosphate Buffer in proportion 65:35 v/v respectively. The optimized columns used are C18 column, Symmetry and Zodiac column. X bridge C18 (4.6×150 mm, 5 µm) particle size was found to be ideal as it gave good peak shape and resolution at 1ml/min flow for Atazanavir and Ritonavir. In this study, the validation of Atazanavir and Ritonavir in API and marketed formulations were performed keeping in accordance with the parameters like system suitability, specificity, linearity, accuracy, precision (reproducibility & repeatability), robustness. The developed stability indicating method is capable for determination of impurities of Atazanavir and Ritonavir in combined tablet dosage form as well as individual dosage forms also. The method has been successfully validated according to ICH guidelines and the results obtained by using RP – HPLC are rapid, accurate and precise. The proposed methods were successfully applied for the analysis of synthetic mixtures and pharmaceutical formulations of Atazanavir and Ritonavir.
Authors and Affiliations
Tadikonda Rama Rao, Ponnapati Srividya,
Keywords
Related Articles
- EP ID EP746805
- DOI 10.47191/ijcsrr/V7-i10-04
- Views 63
- Downloads 0
Development of Low Carbon Cement Ecosystem in Indonesia Using Combine Stakeholder Analysis and Social Network Analysis: In a Perspective of Cement Company
GHG emission remain the main issue for cement industry as well as global GHG emission record. GHG emission was still considered as the main contributor of global warming. Therefore, cement industry has an obligation to d...
Influence of Dividend Payout Rate on Financial Performance of Selected Listed Companies
The purpose of this study was to investigate the effect of dividend payout on financial performance of selected companies listed on the NSE, Kenya. The study was guided by the following specific objectives: Establish the...
Enhancing Asset Performance: Maintenance Strategies for the GBC Pumping System at PT Freeport Indonesia
Focusing on Grasberg Block Caving (GBC) pumping system PT Freeport Indonesia, this study examines the factors of not achieving target and analyze the recommended solution. The research aims to identify the root causes of...
Managing Technical and Vocational Education and Training (TVET) Programmes in a Challenging Economic Environment: Responses of Administrators of Selected TVET Colleges in Yaounde 3, Centre Region, Cameroon
The study employed the survey research design for the collection of data from administrators of selected TVET colleges located in the administrative area of Yaounde 3, Centre Region of Cameroon. The study involved the c...
Strategic Business Adaptations and Growth Opportunities for Cahya Bali Resort in Bali’s Competitive Coastal Environment
This research analyzes the strategy adaptation and growth opportunities for Cahya Bali Resort in a high-competitive property industry in Bali. The research focuses in identifying factors that influence customers’ purchas...