Abstract

A simple, specific, sensitive and validated high-performance thin layer chromatographic (HPTLC) method was developed for the simultaneous analysis of Salbutamol sulphate and Guaiphenesin. Spectro-densitometric scanning-integration was performed at an absorbance wavelength 280 nm. A TLC aluminium sheet pre coated with silica gel 60 F254 was used as the stationary phase. The mobile phase system containing Ethyl acetate: Methanol: Ammonia (25% w/v) (75: 15: 10 v/v) gave a good resolution of Salbutamol sulphate and Guaiphenesin with Rf values of 0.47 and 0.65, respectively. The calibration plot of Salbutamol sulphate exhibited good linear regression relationship (r = 0.9987) over a concentration range of 200-1000 ng/spot. The calibration plot of Guaiphenesin exhibited good polynomial regression relationship (r = 0.9997) over a concentration range of 10-50 μg/spot. Detection and quantitation limit was found to be 70 ng and 100 ng respectively, for Salbutamol sulphate and 30 ng and 50 ng, for Guaiphenesin. The proposed method was used for determination of both drugs in Ventorlin and Asthalin Syrup containing Salbutamol sulphate and Guaiphenesin with satisfactory precision (Intraday) [2.67-4.46% for Salbutamol sulphate and 2.39-4.42% for Guaiphenesin] and accuracy [100.97± 0.50% and 100.45 ± 0.58% RSD, for Salbutamol sulphate and Guaiphenesin respectively]

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