Simultaneous for the estimation of irbesartan (IRBES) and hydrochlorothiazide (HCTZ) in pharmaceutical formulations by UFLC method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
A simple, rapid, sensitive, specific, precise and accurate Ultra-Fast liquid chromatography (UFLC) method was developed for simultaneous determination of Irbesartan and Hydrochlorothiazide in Pharmaceutical formulations. The chromatographic separation was conducted on Shimadzu (Prominence LC 20 UFLC XR) connected with photodiode array (PDA) detector; using ACE column, C18/CN (100 x 4.6 mm, 5 µm) as stationary phase. Isocratic mobile phase consisted of Methanol and 0.1% Phosphoric acid 85% in the ratio of (55:45 v/v); at flow rate of 1.5 ml min-1 was used. An injection volume of 20 µL was used for both Irbesartan and Hydrochlorothiazide. The detection wavelength (λ max) was 225 nm using a diode array detector. Linearity of the method was established over the concentration ranges of 60 – 600 µg ml -1 for Irbesartan, with a retention time of 2.10 minutes and 5 – 50 µg ml -1 for Hydrochlorothiazide, with a retention time of 0.88 minutes. Correlation coefficients were greater than 0.999. The relative standard deviation (RSD) was found to be < 2. The method can be used successfully for simultaneous determination of Irbesartan and Hydrochlorothiazide in pharmaceutical formulations.
Authors and Affiliations
Mohamed A. Amin, Alaa S. Amin, Mounir Z. Saad
Keywords
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- EP ID EP415642
- DOI 10.9790/3008-1305034753.
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