Simultaneous quantification of flavonoids in blood plasma by a high-performance liquid chromatography method after oral administration of Blumea balsamifera leaf extracts in rats

Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 2

Abstract

The leaves of Blumea balsamifera are used as a folk medicine in kidney stone diseases in South-East Asia. Phytochemical investigation revealed leaves contained a number of flavonoids. In view of these, the present work was aimed to quantify and preliminary pharmacokinetic investigation of five flavonoids viz. dihydroquercetin-7,4-dimethyl ether (I), dihydroquercetin-4-methyl ether (II), 5,7,3,5-tetrahydroxyflavanone (III), blumeatin (IV) and quercetin (V) in rat plasma following oral administration (0.5g/Kg) of B. balsamifera leaf extract in rats. Quantification was achieved by using a validated, reproducible high-performance liquid chromatographic method. The mean recoveries of I, II, III, IV and V were 90.6, 93.4, 93.5, 91.2 and 90.3% respectively. The limit of quantification was 25 ng/mL for I and IV, 10 ng/mL for II and III and 100 ng/mL for V respectively. The within day and day-to-day precision for all the compounds were < 10%. The validated HPLC method herein was applied for pharmacokinetic studies and the main pharmacokinetic parameters were: t1/2 (hr) 5.8, 4.3, 2.9, 5.7 and 7.3, Cmax (ng/mL) 594.9, 1542.9 1659.9, 208.9 and 3040.4; Tmax (hr) 4.7, 1.0, 1.0, 3.5 and 2.3; AUC0- (ng hr/mL) 5040, 5893, 9260, 1064 and 27233 for I, II, III, IV and V respectively. The developed method was suitable for pharmacokinetic studies and this preliminary study also revealed significant absorption after oral dosing in rats.

Authors and Affiliations

Fazilatun Nessa, Zhari Ismail, Nornisah Mohamed, Sundram Karupiah

Keywords

Related Articles

 Preliminary phytochemical screening, Antibacterial potential and GC-MS analysis of two medicinal plant extracts

 The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azad...

 Fatty acids, essential oil and phenolics composition of Silybum marianum seeds and their antioxidant activities

 The presentstudydescribes the biochemical evaluation of Silybum marianum seed. The analysis of essential oil composition of Silybum marianum seed by Gas Chromatography-Mass Spectrometry GC-MS showed the presence of...

 Moisturizing effect of stable cream containing Crocus sativus extracts

 The present study is about to prepare stable cream of water-in-oil emulsion containing extracts of Crocus sativus against its base (without extracts) taken as control, to determine its stability on different storag...

Synthesis, characterization and biological activity of some 1, 3, 4-thiadiazol derivatives

Thiadiazoles are their derivatives exhibit a wide variety of pharmacological activities such as Antibacterial and anti-inflammatory. In the present study we have synthesized derivatives some 2,5 substituted 1,3,4- thiadi...

 Screening for toxigenic Escherichia coli in stool samples of diarrhoeal patients by polymerase chain reaction

 Escherichia coli (E. coli) are normal flora of the intestines of most animals, including humans. Most strains are harmless and beneficial to host by preventing the establishment of pathogenic bacteria within the in...

Download PDF file
  • EP ID EP156481
  • DOI -
  • Views 85
  • Downloads 0

How To Cite

Fazilatun Nessa, Zhari Ismail, Nornisah Mohamed, Sundram Karupiah (2013). Simultaneous quantification of flavonoids in blood plasma by a high-performance liquid chromatography method after oral administration of Blumea balsamifera leaf extracts in rats. Pakistan Journal of Pharmaceutical Sciences, 26(2), 375-381. https://europub.co.uk/articles/-A-156481