SIMULTANEOUS RP-HPLC ANALYSIS OF QUERCETIN AND KAEMPFEROL IN DIFFERENT PLANT PARTS OF CISSUS QUADRANGULARIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: To develop a simple, rapid and specific reversed phase High-Performance Liquid Chromatography (HPLC) method for comparative analysis of flavonoids, quercetin and kaempferol, in different plant parts (leaves, stem and roots) of Cissus quadrangularis Linn.Methods: The HPLC method which can be used effectively for separation of components from plants has been developed to perform a comparative analysis of flavonoids, quercetin and kaempferol, in different plant parts (leaves, stem and roots) of Cissus quadrangularis Linn. An endcapped C18 column at 370 nm, and water: acetonitrile (45:55) containing 0.1% o-phosphoric acid as mobile phase was used.Results: Quercetin and kaempferol were well resolved at about 5 min and 7 min respectively. Calibration curves for quercetin and kaempferol were linear in the range of 1-10 µg/ml (R2= 0.999) and 0.5-10 µg/ml (R2= 0.999) respectively. The sensitivity of the method was found to be higher, with a limit of detection (LOD) values of 0.42 μg/ml for kaempferol and 0.48 μg/ml for quercetin and limit of quantification (LOQ) values for kaempferol and quercetin 1.27 μg/ml and 1.47 μg/ml respectively. Percentage recovery values for kaempferol were found to be 99.18, 99.03 and 98.32 for the leaves, stem and root respectively, and for quercetin, they were 99.77, 100.12 and 100.54 for the leaves, stem and root respectively.Conclusion: The developed HPLC method for the analysis of flavonoids has enabled rapid, accurate and reproducible determination. The method can be applied successfully for analysis of quercetin and kaempferol in various plant parts of C. quadrangularis.Keywords: Cissus quadrangularis, HPLC, Kaempferol, Quercetin
Authors and Affiliations
Vandana Jain, Mohammed Shadab Shaikh
ROLE OF SUSPENDED PARTICULATE MATTER IN ANGIOGENESIS EMPLOYING CROWN GALL TUMOR ASSAY
Objective: Aim of the study was to screen the suspended particulate matter for their role in enhancing angiogenesis employing crown gall tumor assay.Methods: Particulate matter (PM10) samples were collected, on glass fib...
28-HOMOCASTASTERONE: A NOVEL DIETARY PHYTO KETO OXYSTEROL MODULATING TESTICULAR STEROID METABOLISM AND LxR mRNA EXPRESSION IN DIABETIC RAT
Objective: Present study aims to investigate 28-homocastasterone (28-HC) influences on testicular tissue in the normal and diabetic rat.Methods: Induction of diabetes was achieved by single peritoneal injection of strept...
HAIR GROWTH PROMOTING ACTIVITY OF CARTHAMUS TINCTORIUS FLORETS EXTRACT-LOADED NANOSTRUCTURED LIPID CARRIERS
Objectives: This work was aimed to formulate, characterize and determine hair growth promoting activity of the Carthamus tinctorius (safflower) florets extract-loaded nano structured lipid carriers (NLC) in C57BL/6 mice....
DETERMINATION OF ANTIOXIDANT CAPACITY IN PLUCHEA INDICA LESS LEAVES EXTRACT AND ITS FRACTIONS
Objective: This study was conducted to determine the DPPH free radical scavenging activity of pluchea extract (PE) and its fraction (ethyl acetate (EAF), n-butanol (BF) and aqueous (AF)) and comparing with the its activi...
CLOUD POINT EXTRACTION, PRECONCENRATION AND SPECTROPHOTOMETRIC DETERMINATION OF COBALT IN WATER SAMPLES
Objective: A new cloud point extraction (CPE) method was developed for pre concentration of trace cobalt (II) in water samples.Methods: The method is based on the complexation reaction of Co (II) with 2-(benzothiazolyl a...