SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP) OF BACILLUS SPECIES USING REC A CANDIDATE GENE APPROACH
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 17
Abstract
The aim of the study was to isolate and characterize Bacillus species, which could be used as probiotic candidates. Initially, Bacillus isolates were collected from milk, cream, fresh tomato and soil. Based on morphological and biochemical tests, 37 Bacillus isolates including 3 standard cultures were subjected to molecular characterization using polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) technique with partial recA gene. The SSCP analysis of 263 bp partial recA amplicons of Bacillus isolates yielded complex SSCP patterns on 8% polyacrylamide gel. On the basis of SSCP band pattern of partial recA PCR products, all the selected isolates were classified into twelve different groups. The SSCP analysis of partial recA PCR products was found to be a powerful tool for illustrating polymorphism or heterogeneity within Bacillus species.
Authors and Affiliations
LOPAMUDRA HALDAR
Keywords
Related Articles
- EP ID EP170520
- DOI -
- Views 79
- Downloads 0
MORPHOMETRIC ANALYSIS USING REMOTE SENSING AND GIS: A CASE STUDY OF TIMMAPUR WATERSHED, RAICHUR DISTRICT, KARNATAKA
The study area is situated in the North-Eastern dry zone of Karnataka at 16º 17´ N latitude and 77º 9´ E longitudes to 16º 23´ N latitude and 77º 18´ E longitudes. The drainage network of watershed was delineated...
VARIATIONS IN STEVIOSIDE AND REBAUDIOSIDE-A CONTENT IN STEVIA REBAUDIANA UNDER DIFFERENT GROWING CONDITIONS
The effects of different growing conditions (open field and polyhouse) were investigated on stevioside and rebaudioside-A content of Stevia rebaudiana plant. The experimental results revealed that stevioside and rebaudio...
GENE ACTION AND COMBINING ABILITY ANALYSIS IN CMS BASED HYBRID RICE FOR GRAIN YIELD AND QUALITY ATTRIBUTING TRAITS
Study of combining ability and genetic behavior of the parents are essential to search newer hybrid combinations with better grain quality features. An experiment was conducted on twenty one crosses which were generated...
ASSESSMENT OF KNOWLEDGE GAP AND CONSTRAINTS OF POTATO GROWERS IN TAWANG DISTRICT OF ARUNACHAL PRADESH
Potato is grown as cash crop in North East Hill (NEH) region of India. The current study was planned in Tawang district of Arunachal Pradesh to study the knowledge level, gap in knowledge of farmers and constraints in po...
HERMAPHRODITISM IN A CROSSBRED PIG : A CASE STUDY
A crossbred piglet of Tamworth x Deshi variety was born in ICAR Research Centre, Ranchi with both male and female genitalia. Birth weight of piglet was 1.11 gm. There was scrotal sac, testis and vagina in inguinal region...