Some progresses in the electrogenerated chemiluminescence<br /> techniques of Ru(bpy)32+<br />
Journal Title: Science Paper Online - Year 2007, Vol 2, Issue 8
Abstract
Electrogenerated chemiluminescence (ECL), which is a chemiluminescence produced directly or indirectly as a result of electrochemical reactions, has proved to be a sensitive and selective detection method with a wide linear range partly due to the electrochemical excitation during ECL reaction and no external excitation light source, which gives an extremely low background signal without expensive instruments. Tris(2,2’-bipyridyl)ruthenium(Ⅱ) Ru(bpy)32+ and its derivatives-based ECL systems have shown great importance for the analysis of various compounds such as oxalate, amino acids, aliphatic amines, cyclic amines, aromatic amines, pharmaceuticals or proteins due to its ability to undergo ECL in aqueous solutions and its good chemical, electrochemical, and photochemical stability. The ability of ruthenium complexes to produce ECL with a large number of analytes often requires separation steps prior to detection. The ECL of Ru(bpy)32+ is often used as a detection method in flowing systems such as FIA, HPLC, CE and MCE etc. Since Ru(bpy)32+ can be electrochemically regenerated, there is currently a strong interest in immobilizing this species onto the electrode surface to form ECL detectors. There is no need to continually deliver this reagent to the electrode surface, thus simplifying instrumentation, removing the need for an extra-pump, avoiding the problems of both sample dilution and band broadening and largely reducing the consumption of the expensive reagents. We have fabricated several novel solid-state ECL sensors with high sensitivity and good stability. More importantly, several devices such as FIA, CE and MCE in combination with the solid-state ECL detectors have been constructed in our laboratory. Based on our research works, a review related some new progresses in ECL techniques with Ru(bpy)32+ has been presented.
Authors and Affiliations
Shounian Ding, Jingjuan Xu , Hongyuan Chen
Keywords
Related Articles
- EP ID EP102239
- DOI -
- Views 113
- Downloads 0
新型含B耐火钢的性能及组织特征研究
应用光学显微镜(OM)、场发射扫描电子电镜(SEM)、透射电镜(TEM)等技术，对含B耐火钢试验钢的力学性能和常温、600℃回火、高温拉伸状态的组织结构进行了研究，分析了合金元素B、V在耐火钢中的作用。研究结果表明：①合金元素B促进贝氏体的形...
Study on mechanical behavior of frame-supported transfer beam
To deeply understand the transfer beam’s stress characteristics and to propose the criterion of the eccentric-tensional-beam, tests of two one-forth-scale specimens under cyclic load reversals were implemented. Based on...
智能化微囊膜研究新进展
智能化控制释放系统一直是国内外科技工作者的研究热点，迄今已经研究报道了多种具有不同工作原理的环境感应型智能化微囊膜。本文介绍作者近几年在温度感应型、葡萄糖浓度感应型以及分子识别型等环境感应型智能化微囊膜方面取得的成果，并...
苯在Ru-Zn/Ru(0001)合金表面上的吸附<br /> ——密度泛函研究<br />
为了理解苯在Ru-Zn合金催化剂上的部分加氢催化机理，采用密度泛函方法对苯在Ru-Zn/Ru(0001)面上的吸附进行理论研究。计算结果表明，当苯的碳原子或者共轭双键直接位于表面Zn原子的atop位时，其吸附热较Ru(0001)面上的类似吸附状态下降约6...
两步升温法酸催化潲水油制备生物柴油
回收的潲水油经适当的预处理后，采用两步升温法在浓硫酸催化作用下与甲醇进行酯交换反应制备生物柴油。考察了重要因素对反应的影响，得出了利用潲水油制备生物柴油的最佳条件：催化剂体积为潲水油体积的5%，醇油摩尔比为20:1，反应时间为...