Some protozoa are agent of dangerous zoonosis

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Some protozoa are agent of dangerous zoonosis

Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue

Abstract

Introduction. The role of giardia and cryptosporidia in the pathology of gastrointestinal diseases of animals and humans for a long time was at subject of discussion and still is. Currently most of scientists believe that giardiasis is a protozoa disease of animals and humans with a variety of manifestations: latent parasitosis, in clinical infection or acute illness with clinical manifestations of diarrhea of varying difficulty levels the presence of allergic and neurotic phenomena. By the level of sensitivity to cryptosporidia infection, scientists divided animals in two groups. The first group was susceptible to animals that, did not show any clinical signs after infection (dogs, cats, mice, rats, rabbits, mulls). Representatives of the second group - calves, lambs, piglets, young birds (chickens, turkeys), monkeys, as well as human get sick after infection manifesting certain clinical signs: diarrhea of varying difficulti levels, anorexia, exocytosis. The goal of the work. To carry out epizootic monitoring of gіardiasis and cryptosporidiosis of dogs of Kharkiv and Kharkiv oblast, with confirmed carrier stage by coproscopic methods, ELISA, PCR, to determine the threat of giardiasis the causative agent of dogs ryarding human. Materials and methods. 216 dogs from the Center for Animal Welfare and the Cynological Center were examined. 124 animals were examined using the coupling method, 92 dogs samples were tested by ELISA and PCR methods. A technique for infecting puppies with coproscopic material from humans was developed and studies on the experimental infection of puppies by human giardia were carried out. Results of research and discussion. According to the results of 124 dogs samples studies on giardiasis and cryptosporidiosis, using new coproscopic method, the highest invasion extensiveness concerning giardiasis was registered in 48% cases and 44% of cases concerning cryptosporidiosis, in animals of 2 to 5 months old age. The study of 92 dogs using the ELISA method showed positive results for giardiasis in 74 samples, which was 80,4%, and for cryptosporidiosis in 70 (76,1%). According to the research of feces from 92 dogs using PCR method, 39 (42,4%) positive samples were confirmed regarding Giardia duodenalis, 22 (23,9%) for Cryptosporidium parvum and mixed infection of Giardia duodenalis + Cryptosporidium parvum in 14 cases (15,2%). Studies on the detection of Giardia duodenalis genotypes by PCR in humans and in experimentally infected puppies were conducted. Conclusions and prospects for further research. Studies indicated the carriage of giardia and cryptosporidia in dogs, both monoinvasia and mixed infection in Kharkiv and Kharkiv oblast, and circulation of the Zoonotic/A genotype in a human and dogs. In the future, studies focused on the spread of diseases and the detection of genotypes in other animals will be conducted.

Authors and Affiliations

Yuriy Prykhodko, Volodymyr Ponomarenko, Viktoriy Bulavina

Keywords

dogs protozoa giardia cryptosporidia genotypes Zoonotic/A Zoonotic/B IFA PCR

EP ID EP406142
DOI 10.31073/vet_biotech32(2)-54
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