Species relationships among wild and cultivated Abelmoschus Medik., (Malvaceae) species as reveled by molecular markers
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 1
Abstract
The genus Abelmoschus is represented by more than 10 species which shows wide distribution over the diverse agro−climatic conditions of Indian subcontinent. In the present study, total 60 accessions of eleven Abelmoschus species were subjected to estimate genetic diversity and species relationships. Out of 200 primers 21 primers was found to be considerable for cluster analysis. All 21 primers produced total 321 amplicons with ranges from nine (OPA-5) to nineteen (105, 966) and average number of amplicons detected were 15.3 per primer. Out of the 321 amplicons amplified, 301 were polymorphic showing 93.77% polymorphism with average of 14.3 per primer. Among the studied species, high genetic diversity was observed in A. manihot subsp. tetraphyllus var. tetraphyllus (0.1711), whereas lowest genetic diversity was observed in A. manihot subsp. tetraphyllus var. pungens. Among the studied species highest (0.9095) similarity index was observed between A. esculentus and A. caillei whereas A. palianus and A. crinitus exhibited lowest (0.5149) similarity index. The UPGMA based cluster analysis clearly distinguishes the Abelmoschus species into three major clusters – I, II and III. This study validate the utility of RAPD markers as a reliable tool for phylogenetic relationships as well as effective characterization of Abelmoschus genetic resources.
Authors and Affiliations
Pravin Patil, Shrikant Sutar, Kangila Venkataraman Bhat
Keywords
Related Articles
- EP ID EP542480
- DOI -
- Views 63
- Downloads 0
Cytochemical characterisation of photochemically formed, self-sustaining, abiogenic, protocell-like, supramolecular assemblies “Jeewanu”
Sunlight exposed sterilised aqueous mixture of some inorganic and organic substances reported by Bahadur and Ranganayaki provided an optimal physico-chemical environment for the self-assembly of molecules and photochemic...
Analysis of some physicochemical parameters and their effect on the productivity of fishes in two different types of aquatic bodies of Unamgaon and Seipargaon of Patharkandi block of Karimganj district, Assam, India
Water quality is determined by various physicochemical and biological factors, as they may directly or indirectly affect its quality and consequently its suitability for the distribution and production of fish and other...
Significance and Management of Chickpea Wilt/root rot and Future Prospects in Ethiopia. A Review
Chickpea production and productivity in Ethiopia has recently declined and high potential yield gaps because of several abiotic and biotic factors. The average chickpea yield in Ethiopia is usually below 2 t/ha although...
Encapsulation of Lactic acid bacteria in calcium alginate beads for higher bacteriocin production
Lactic acid bacteria (LAB) strains LAB -A, LAB -B and LAB-C isolated from batter of idli, very popular fermented food of south India and identified to species level using 16 S rRNA sequencing as Pediococcus acidilactici...
An exploration of species composition of vertebrate fauna of Orchha Wildlife Sanctuary, Central India
This research is an exploration of vertebrate fauna of the Orchha Wildlife Sanctuary (OWS) in central India. The study consolidates information from field surveys. A total of 15 fish (6 families), 6 amphibians (3 familie...