Species Specific Activity of Digestive Enzymes in Two Freshwater Prawns Macrobrachium rosenbergii and Macrobrachium malcolmsonii Juveniles
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2016, Vol 10, Issue 3
Abstract
Aim: The activity pattern of digestive enzymes (protease, amylase, lipase and cellulase) was studied in two species of freshwater prawns Macrobrachium rosenbergii and Macrobrachium malcolmsonii juveniles. Study Design: Digestive capacity of individual species may vary. In order to see whether the activities of protease, amylase, lipase and cellulase in two species of freshwater prawns are more or less similar or not under a prescribed methodology. Methodology: The hepatopancreas of prawns was blended and suspended in 10 mM phosphate buffer. After centrifugation at 15,000 rpm for 5 min, the supernatant was collected and used as crude enzyme source for the assays of protease, amylase, lipase and cellulase activities by adopting the standard methods. Results: The activity patterns of protease, amylase, lipase and cellulase were more or less similar in both the species studied. However, M. malcolmsonii showed little higher activities than that of M. rosenbergii. Conclusion: There were no clear cut species specific activities of digestive enzymes observed between M. rosenbergii and M. malcolmsonii.
Authors and Affiliations
Annamalai Asaikkutti, Periyakali Saravana Bhavan, Karuppaiya Vimala, Madhayan Karthik
Keywords
Related Articles
- EP ID EP351756
- DOI 10.9734/JABB/2016/29404
- Views 88
- Downloads 0
Isolation and Optimization of Lipase Producing Microorganism from Degrading Palm (Elaeis guineensis) Kernel Nut Oil
Aim: Most commercially useful lipases are of microbial origin. The increasing tendency of its market shows the importance to search new microbial resources to produce these enzymes. Study Design: The study is designed at...
Phylogeny and Bioinformatics Study on Leaf Tissue Genes for Selected Elite Cultivars of Plantain (Musa paradisiaca L.) in the Rain Forest Ecology of Nigeria
Aims: To show that phylogenetic and bioinformatics study could be very crucial to the survival and adaptation of elite plantain species that are threatened by rapid changes in environment, urbanization, genetic erosion a...
Optimization of Conditions for Biosorption of Chromium and Cadmium by Shell of Anodontoides ferussacianus
The biosorption of chromium [Cr (VI)] and cadmium [Cd (II)] ions using dead biomass of bivalve mollusk shell, Anodontoides ferussacianus was studied. Effect of various physico-chemical parameters was determined. Optimum...
Comparative Analysis of Genetic Structure and Diversity of Sorghum (Sorghum bicolor L.) Local Farmer’s Varieties from Sudan
Aims: Investigate the genetic diversity and structure of 50 Sorghum accessions from 10 different regions in Sudan and one from the county of Central Equatoria in the Republic of South Sudan, by screening 40 RAPD and 10 I...
Optimization of Cultural Conditions for Production of Extracellular Polysaccharide by Halomonas xianhensis SUR308 Using Weighted Response Surface Methodology
Optimization of different physico-chemical and cultural parameters such as temperature, pH, incubation time and inoculum dose along with variation of NaCl, glucose and casein hydrolysate concentrations were carried out i...